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Literature summary for 2.7.1.90 extracted from

  • Groenewald, J.H.; Botha, F.C.
    Molecular and kinetic characterisation of sugarcane pyrophosphate: fructose-6-phosphate 1-phosphotransferase and its possible role in the sucrose accumulation phenotype (2007), Funct. Plant Biol., 34, 517-525.
    View publication on PubMed
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate increases the Vmax for the forward and reverse reactions by similar amounts Saccharum hybrid cultivar

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 diphosphate
-
 Saccharum hybrid cultivar
0.039
-
 D-fructose 1,6-bisphosphate
-
 Saccharum hybrid cultivar
0.15
-
 D-fructose 6-phosphate
-
 Saccharum hybrid cultivar
0.46
-
 phosphate
-
 Saccharum hybrid cultivar

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 63200 + x * 58000, the enzyme exists as dimer, tetramer or octamer, fructose 2,6-bisphosphate influences this aggregation state Saccharum hybrid cultivar
63200
-
 x * 63200 + x * 58000, the enzyme exists as dimer, tetramer or octamer, fructose 2,6-bisphosphate influences this aggregation state Saccharum hybrid cultivar
129000
-
 dimer, gel filtration Saccharum hybrid cultivar
245000
-
 tetramer, gel filtration Saccharum hybrid cultivar
511000
-
 octamer, gel filtration Saccharum hybrid cultivar

Organism

Organism UniProt Comment Textmining
Saccharum hybrid cultivar
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharum hybrid cultivar

Source Tissue

Source Tissue Comment Organism Textmining
internode the amount of pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) activity in sugarcane internodal tissue is inversely correlated with sucrose content Saccharum hybrid cultivar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.23
-
-
 Saccharum hybrid cultivar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Saccharum hybrid cultivar phosphate + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 63200 + x * 58000, the enzyme exists as dimer, tetramer or octamer, fructose 2,6-bisphosphate influences this aggregation state Saccharum hybrid cultivar

Synonyms

Synonyms Comment Organism
pyrophosphate: fructose-6-phosphate 1-phosphotransferase
-
 Saccharum hybrid cultivar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 8 glycolytic and gluconeogenic reactions. D-fructose 2,6-bisphosphate shifts the pH optimum for the forward reaction to a slightly more basic pH Saccharum hybrid cultivar