Literature summary for 2.7.1.90 extracted from

Deng, Z.; Wang, X.; Kemp, R.G.
Site-directed mutagenesis of the fructose 6-phosphate binding site of the pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica (2000), Arch. Biochem. Biophys., 380, 56-62.

Search for more literature for 2.7.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutants in Escherichia coli DF1020	Entamoeba histolytica

Protein Variants

Protein Variants	Comment	Organism
H415A	site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate and decreased Km for diphosphate	Entamoeba histolytica
H416A	site-directed mutagenesis, reduced activity, slightly increased Km for fructose 6-phosphate	Entamoeba histolytica
K408A	site-directed mutagenesis, activity , decreased Km for diphosphate	Entamoeba histolytica
R377A	site-directed mutagenesis, increased Km values for fructose 6-phosphate and diphosphate	Entamoeba histolytica
R405A	site-directed mutagenesis, increased activity, kinetic properties similar to the wild-type	Entamoeba histolytica
R423A	site-directed mutagenesis, decrease of kcat by 10000fold, 10fold increased Km for diphosphate, 126fold increased Km for fructose 6-phosphate	Entamoeba histolytica
S392A	site-directed mutagenesis, activity and kinetic properties similar to the wild-type	Entamoeba histolytica
Y420A	site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate	Entamoeba histolytica
Y420F	site-directed mutagenesis, reduced activity, increased Km for fructose 6-phosphate, decreased Km for diphosphate	Entamoeba histolytica
Y420H	site-directed mutagenesis, reduced activity, increased Km values for fructose 6-phosphate and diphosphate	Entamoeba histolytica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km values for substrates of mutant enzymes	Entamoeba histolytica
0.04	-	diphosphate	wild-type, pH 7.2, 30°C	Entamoeba histolytica
0.085	-	D-fructose 6-phosphate	wild-type, pH 7.2, 30°C	Entamoeba histolytica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Entamoeba histolytica

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate	Arg423 and Tyr 420 are involved in substrate binding and catalytic mechanism	Entamoeba histolytica	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	specific for diphosphate as phosphor donor	Entamoeba histolytica	phosphate + D-fructose 1,6-bisphosphate	-	r

Synonyms

Synonyms	Comment	Organism
PPi-dependent phosphofructokinase	-	Entamoeba histolytica
PPi-PFK	-	Entamoeba histolytica
pyrophosphate-dependent phosphofructokinase	-	Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kcat values of mutant enzymes	Entamoeba histolytica
330	-	diphosphate	wild-type, pH 7.2, 30°C	Entamoeba histolytica
330	-	D-fructose 6-phosphate	wild-type, pH 7.2, 30°C	Entamoeba histolytica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Entamoeba histolytica

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Release 2023.1 (January 2023)