Literature summary for 2.7.1.90 extracted from

Ding, Y.H.; Ronimus, R.S.; Morgan, H.W.
Purification and properties of the pyrophosphate-dependent phosphofructokinase from Dictyoglomus thermophilum Rt46 B.1 (1999), Extremophiles, 3, 131-137.

Search for more literature for 2.7.1.90

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	complete inhibition at 1 mM	Dictyoglomus thermophilum
Fe3+	84% inhibition at 0.1 mM	Dictyoglomus thermophilum
Mn2+	46% inhibition at 0.1 mM	Dictyoglomus thermophilum
Se4+	75% inhibition at 0.1 mM	Dictyoglomus thermophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	diphosphate	pH 6.0, 50°C	Dictyoglomus thermophilum
0.23	-	D-fructose 6-phosphate	pH 6.0, 50°C	Dictyoglomus thermophilum
2.9	-	D-fructose 1,6-bisphosphate	pH 7.2, 50°C	Dictyoglomus thermophilum
4.3	-	phosphate	pH 7.2, 50°C	Dictyoglomus thermophilum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Dictyoglomus thermophilum	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates 120% at 0.1 mM	Dictyoglomus thermophilum
Co2+	-	Dictyoglomus thermophilum
divalent cations	activates 120% at 0.1 mM	Dictyoglomus thermophilum
Fe2+	activates 120% at 0.1 mM	Dictyoglomus thermophilum
K+	activates 140% at 10 mM	Dictyoglomus thermophilum
Mg2+	optimal at 0.5-3.5 mM	Dictyoglomus thermophilum
Na+	activates 135% at 10 mM	Dictyoglomus thermophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000, SDS-PAGE	Dictyoglomus thermophilum
65000	-	gel filtration	Dictyoglomus thermophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	Dictyoglomus thermophilum	-	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	Dictyoglomus thermophilum Rt46 B.1	-	phosphate + D-fructose 1,6-bisphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Dictyoglomus thermophilum	-	-	-
Dictyoglomus thermophilum Rt46 B.1	-	-	-

Oxidation Stability

Oxidation Stability	Organism
not sensitive to O2	Dictyoglomus thermophilum

Purification (Commentary)

Purification (Comment)	Organism
231fold, to homogeneity	Dictyoglomus thermophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
24.4	-	purified enzyme	Dictyoglomus thermophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(polyphosphate)n + D-fructose 6-phosphate	n: 12-18	Dictyoglomus thermophilum	(polyphosphate)n-1 + D-fructose 1,6-bisphosphate	-	r
(polyphosphate)n + D-fructose 6-phosphate	75% of activity with diphosphate	Dictyoglomus thermophilum	(polyphosphate)n-1 + D-fructose 1,6-bisphosphate	-	r
(polyphosphate)n + D-fructose 6-phosphate	n: 12-18	Dictyoglomus thermophilum Rt46 B.1	(polyphosphate)n-1 + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	-	Dictyoglomus thermophilum	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	equilibrium lies in forward direction	Dictyoglomus thermophilum	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate	Dictyoglomus thermophilum	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	-	Dictyoglomus thermophilum Rt46 B.1	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	equilibrium lies in forward direction	Dictyoglomus thermophilum Rt46 B.1	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate	Dictyoglomus thermophilum Rt46 B.1	phosphate + D-fructose 1,6-bisphosphate	-	r
triphosphate + D-fructose 6-phosphate	82% of activity with diphosphate	Dictyoglomus thermophilum	diphosphate + D-fructose 1,6-bisphosphate	-	r
triphosphate + D-fructose 6-phosphate	82% of activity with diphosphate	Dictyoglomus thermophilum Rt46 B.1	diphosphate + D-fructose 1,6-bisphosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 37000, SDS-PAGE	Dictyoglomus thermophilum

Synonyms

Synonyms	Comment	Organism
PPi-dependent phosphofructokinase	-	Dictyoglomus thermophilum
PPi-PFK	-	Dictyoglomus thermophilum
pyrophosphate-dependent phosphofructokinase	-	Dictyoglomus thermophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Dictyoglomus thermophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	half-life: 150 min	Dictyoglomus thermophilum
90	-	half-life: 10 min	Dictyoglomus thermophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.7	6.3	50°C, forward reaction	Dictyoglomus thermophilum
7	7.5	50°C, reverse reaction	Dictyoglomus thermophilum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Dictyoglomus thermophilum	-	-	4.4

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)