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Literature summary for 2.7.1.86 extracted from

  • Turner, W.L.; Waller, J.C.; Snedden, W.A.
    Identification, molecular cloning and functional characterization of a novel NADH kinase from Arabidopsis thaliana (thale cress) (2005), Biochem. J., 385, 217-223.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
diphosphate mixed inhibitor both to NADH and ATP Arabidopsis thaliana
p-chloromercuribenzoate 0.01 mM, 42% residual activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 NADH pH 7.9, 25°C Arabidopsis thaliana
0.042
-
 NADH pH 7.9, 25°C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+ Arabidopsis thaliana
0.062
-
 ATP pH 7.9, 25°C Arabidopsis thaliana
0.062
-
 ATP pH 7.9, 25°C, cosubstrate NADH Arabidopsis thaliana
0.156
-
 UTP pH 7.9, 25°C, cosubstrate NADH Arabidopsis thaliana
0.461
-
 GTP pH 7.9, 25°C, cosubstrate NADH Arabidopsis thaliana
0.759
-
 CTP pH 7.9, 25°C, cosubstrate NADH Arabidopsis thaliana
2.39
-
 NAD+ pH 7.9, 25°C Arabidopsis thaliana
2.39
-
 NAD+ pH 7.9, 25°C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+ Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol putative Arabidopsis thaliana 5829
-
additional information not: mitochondrial Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ far less active than Mg2+ and Mn2+ Arabidopsis thaliana
Ca2+ 8% of the activity with Mg2+ Arabidopsis thaliana
Mg2+ Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity. Km-value Mg2+, 1.16 mM Arabidopsis thaliana
Mg2+ required, Km-value 1.16 mM Arabidopsis thaliana
Mn2+ Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity Arabidopsis thaliana
Mn2+ 28% of the activity with Mg2+ Arabidopsis thaliana
Zn2+ far less active than Mg2+ and Mn2+ Arabidopsis thaliana
Zn2+ 4% of the activity with Mg2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Arabidopsis thaliana
78000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
-
 first discovery in plants
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Arabidopsis thaliana
-
cell suspension culture
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.1
-
 NAD+ as substrate, pH 7.9, 25°C Arabidopsis thaliana
15.6
-
 pH 7.9, 25°C, substrate NAD+ Arabidopsis thaliana
40.1
-
 NADH as susbtrate, pH 7.9, 25°C Arabidopsis thaliana
40.1
-
 pH 7.9, 25°C, substrate NADH Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+ NADH is preferred 100fold over NAD+ Arabidopsis thaliana ADP + NADP+
-
 ?
ATP + NADH preferentially phosphorylates reduced NAD+ but can use oxidized NAD+. ATP is preferred phophoryl donor but UTP, GTP, and CTP may substitute with lower activity Arabidopsis thaliana ADP + NADPH
-
 ?
ATP + NADH NADH is preferred 100fold over NAD+ Arabidopsis thaliana ADP + NADPH
-
 ?
CTP + NADH ATP is preferred substrate Arabidopsis thaliana CDP + NADPH
-
 ?
GTP + NADH ATP is preferred substrate Arabidopsis thaliana GDP + NADPH
-
 ?
additional information diphosphate or polyphosphate is not used as a phosphoryl donor Arabidopsis thaliana ?
-
 ?
additional information no substrate: diphosphate, polyphosphate Arabidopsis thaliana ?
-
 ?
UTP + NADH ATP is preferred substrate Arabidopsis thaliana UDP + NADPH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
diphosphopyridine nucleotide (reduced) kinase
-
 Arabidopsis thaliana
NAD(H) kinase
-
 Arabidopsis thaliana
NADHK
-
 Arabidopsis thaliana
NADK3
-
 Arabidopsis thaliana
nicotinamide adenine dinucleotide (reduced) kinase
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Arabidopsis thaliana