Application | Comment | Organism |
---|---|---|
medicine | ethanolamine kinase could be a target for antimalerial therapy | Plasmodium falciparum 3D7 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
into the vector pET24b for expression in Escherichia coli BL21DE3-pRIL cells | Plasmodium falciparum 3D7 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-1-butanol | analogue of ethanolamine, selective inhibition | Plasmodium falciparum | |
2-amino-1-butanol | - |
Plasmodium falciparum 3D7 | |
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | SAR97276 | Plasmodium falciparum 3D7 | |
hemicholinium-3 | - |
Plasmodium falciparum | |
SAR97276 | bis-thiazolium compound designed as a choline analogue, affects ethanolamine kinase and choline kinase activities similarly. Inhibition is competitive and correlates with the impairment of cellular phosphatidylcholine biosynthesis | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.458 | - |
ethanolamine | pH 8.0, 37°C | Plasmodium falciparum | |
0.4757 | - |
ethanolamine | - |
Plasmodium falciparum 3D7 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Plasmodium falciparum 3D7 | 5737 | - |
cytoplasm | of Plasmodium falciparum | Plasmodium falciparum | 5737 | - |
additional information | protein expression levels increase during erythrocytic development | Plasmodium falciparum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
determined by SDS-PAGE and Western Blot analysis | Plasmodium falciparum 3D7 |
109000 | - |
determined by gel filtration | Plasmodium falciparum 3D7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | Plasmodium falciparum 3D7 | - |
ADP + O-phosphoethanolamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IIB7 | 3D7 | - |
Plasmodium falciparum 3D7 | Q8IIB7 | - |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap chelating and a gel filtration column | Plasmodium falciparum 3D7 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
pH 8.0, 37°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | - |
Plasmodium falciparum 3D7 | ADP + O-phosphoethanolamine | - |
? | |
ATP + ethanolamine | - |
Plasmodium falciparum | ADP + O-phosphoethanolamine | - |
? | |
additional information | no substrate: choline | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Plasmodium falciparum 3D7 |
Synonyms | Comment | Organism |
---|---|---|
ethanolamine kinase | - |
Plasmodium falciparum 3D7 |
PfEK | - |
Plasmodium falciparum 3D7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Plasmodium falciparum 3D7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Plasmodium falciparum 3D7 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum 3D7 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | - |
Plasmodium falciparum 3D7 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.46 | - |
pH 8.0, 37°C | Plasmodium falciparum | hemicholinium-3 | |
0.46 | - |
Plasmodium falciparum-endogenous ethanolamine kinase | Plasmodium falciparum 3D7 | 2-amino-1-butanol | |
0.61 | - |
recombinant protein | Plasmodium falciparum 3D7 | 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | |
0.79 | - |
pH 8.0, 37°C | Plasmodium falciparum | SAR97276 | |
0.79 | - |
Plasmodium falciparum-endogenous ethanolamine kinase | Plasmodium falciparum 3D7 | 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | |
1.36 | - |
pH 8.0, 37°C | Plasmodium falciparum | 2-amino-1-butanol | |
1.36 | - |
recombinant protein | Plasmodium falciparum 3D7 | 2-amino-1-butanol |
Organism | Comment | Expression |
---|---|---|
Plasmodium falciparum 3D7 | expression levels increases during erythrocytic development | up |