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Literature summary for 2.7.1.82 extracted from

  • Gibellini, F.; Hunter, W.N.; Smith, T.K.
    Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases (2008), Biochem. J., 415, 135-144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0184
-
ethanolamine pH 7.8 Trypanosoma brucei
0.0206
-
ATP pH 7.8 Trypanosoma brucei
0.0314
-
choline
-
Trypanosoma brucei
0.219
-
ATP pH 7.8 Trypanosoma brucei
2.56
-
ethanolamine pH 7.8 Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49100
-
2 * 49100, calculated, 2 * 51655, MALDI-TOF Trypanosoma brucei
51655
-
2 * 49100, calculated, 2 * 51655, MALDI-TOF Trypanosoma brucei
70140
-
2 * 70140, calculated, 2 * 72614, MALDI-TOF Trypanosoma brucei
72614
-
2 * 70140, calculated, 2 * 72614, MALDI-TOF Trypanosoma brucei
100000
-
gel filtration Trypanosoma brucei
140000
-
gel filtration Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q386V0 isoform EK1
-
Trypanosoma brucei Q57W59 isoform EK2, bifunctional choline kinase/ethanolamine kinase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-1-aminopropan-2-ol 52.4% of the activity with ethanolamine Trypanosoma brucei ADP + (R)-1-aminopropyl 2-phosphate
-
?
ATP + (R)-1-aminopropan-2-ol 7.2% of the activity with choline Trypanosoma brucei ADP + (R)-1-aminopropyl 2-phosphate
-
?
ATP + (R)-2-aminobutan-1-ol 102% of the activity with ethanolamine Trypanosoma brucei ADP + (R)-2-aminobutyl phosphate
-
?
ATP + (R)-2-aminobutan-1-ol 130% of the activity with choline Trypanosoma brucei ADP + (R)-2-aminobutyl phosphate
-
?
ATP + (R)-2-aminopropan-1-ol 108% of the activity with ethanolamine Trypanosoma brucei ADP + (R)-2-aminopropyl phosphate
-
?
ATP + (R)-2-aminopropan-1-ol 110% of the activity with choline Trypanosoma brucei ADP + (R)-2-aminopropyl phosphate
-
?
ATP + (S)-1-aminopropan-2-ol 1.4% of the activity with choline Trypanosoma brucei ADP + (S)-1-aminopropyl 2-phosphate
-
?
ATP + (S)-1-aminopropan-2-ol 6.1% of the activity with ethanolamine Trypanosoma brucei ADP + (S)-1-aminopropyl 2-phosphate
-
?
ATP + (S)-2-aminobutan-1-ol 114% of the activity with ethanolamine Trypanosoma brucei ADP + (S)-2-aminobutyl phosphate
-
?
ATP + (S)-2-aminobutan-1-ol 41% of the activity with choline Trypanosoma brucei ADP + (S)-2-aminobutyl phosphate
-
?
ATP + (S)-2-aminopropan-1-ol 22.4% of the activity with choline Trypanosoma brucei ADP + (S)-2-aminopropyl phosphate
-
?
ATP + (S)-2-aminopropan-1-ol 94% of the activity with ethanolamine Trypanosoma brucei ADP + (S)-2-aminopropyl phosphate
-
?
ATP + 2-amino-2-methylpropan-1-ol 25.6% of the activity with ethanolamine Trypanosoma brucei ADP + 2-amino-2-methylpropan-1-ol phosphate
-
?
ATP + 2-amino-2-methylpropan-1-ol 46% of the activity with choline Trypanosoma brucei ADP + 2-amino-2-methylpropan-1-ol phosphate
-
?
ATP + 3-aminopropan-1-ol 37.5% of the activity with ethanolamine Trypanosoma brucei ADP + 3-aminopropyl 1-phosphate
-
?
ATP + 3-aminopropan-1-ol 44.5% of the activity with choline Trypanosoma brucei ADP + 3-aminopropyl 1-phosphate
-
?
ATP + 4-aminobutan-1-ol 60% of the activity with choline Trypanosoma brucei ADP + 4-aminobutyl 1-phosphate
-
?
ATP + choline
-
Trypanosoma brucei ADP + choline phosphate
-
?
ATP + diethanolamine
-
Trypanosoma brucei ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate 139% of the activity with choline ?
ATP + diethanolamine
-
Trypanosoma brucei ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate 88% of the activity with ethanolamine ?
ATP + ethanolamine
-
Trypanosoma brucei ADP + O-phosphoethanolamine
-
?
ATP + ethanolamine 33% of the activity with choline Trypanosoma brucei ADP + O-phosphoethanolamine
-
?
ATP + N,N-diethylethanolamine
-
Trypanosoma brucei ADP + N,N-diethyl-O-phosphoethanolamine 156% of the activity with choline ?
ATP + N,N-diethylethanolamine
-
Trypanosoma brucei ADP + N,N-diethyl-O-phosphoethanolamine 47% of the activity with ethanolamine ?
ATP + N,N-dimethylethanolamine
-
Trypanosoma brucei ADP + N,N-dimethyl-O-phosphoethanolamine 153% of the activity with choline ?
ATP + N,N-dimethylethanolamine
-
Trypanosoma brucei ADP + N,N-dimethyl-O-phosphoethanolamine 76% of the activity with ethanolamine ?
ATP + N-ethylethanolamine
-
Trypanosoma brucei ADP + N-ethyl-O-phosphoethanolamine 150% of the activity with choline ?
ATP + N-ethylethanolamine
-
Trypanosoma brucei ADP + N-ethyl-O-phosphoethanolamine 97% of the activity with ethanolamine ?
ATP + N-methylethanolamine 106% of the activity with ethanolamine Trypanosoma brucei ADP + N-methyl-O-phosphoethanolamine
-
?
ATP + N-methylethanolamine 135% of the activity with choline Trypanosoma brucei ADP + N-methyl-O-phosphoethanolamine
-
?
additional information no choline kinase activity Trypanosoma brucei ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49100, calculated, 2 * 51655, MALDI-TOF Trypanosoma brucei
dimer 2 * 70140, calculated, 2 * 72614, MALDI-TOF Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.56
-
ethanolamine pH 7.8 Trypanosoma brucei
12.9
-
ethanolamine pH 7.8 Trypanosoma brucei