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Literature summary for 2.7.1.82 extracted from

  • Kersting, M.C.; Choi, H.S.; Carman, G.M.
    Regulation of the yeast EKI1-encoded ethanolamine kinase by inositol and choline (2004), J. Biol. Chem., 279, 35353-35359.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme fused to the lacZ reporter gene in several yeast strains and in Escherichia coli strain DH5alpha, expression analysis in presence of inositol and choline in correlation to wild-type and mutated promotor Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Inositol decreases gene EKI1 expression, involving the transcription factors Ino2p, Ino4p, and Opi1p, the UASINO element of the EKI promotor is required for the repressive regulation, the inhibitory effect on the enzyme is correlated with a decrease of ethanolamine incorporation into CDP-ethanolamine pathway intermediates and into phosphatidylethanolamine and phosphatidylcholine Saccharomyces cerevisiae
additional information enzyme expression is not affected by addition of choline Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ethanolamine Saccharomyces cerevisiae the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview ADP + O-phosphoethanolamine
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
gene EKI1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ethanolamine
-
Saccharomyces cerevisiae ADP + O-phosphoethanolamine
-
?
ATP + ethanolamine the enzyme and the phospholipid phosphatidylethanolamine biosynthesis are regulated by inositol, Kennedy pathway overview Saccharomyces cerevisiae ADP + O-phosphoethanolamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae