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Literature summary for 2.7.1.82 extracted from

  • Wharfe, J.; Harwood, J.L.
    Lipid metabolism in germinating seeds. Purification of ethanolamine kinase from soya bean (1979), Biochim. Biophys. Acta, 575, 102-111.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ in presence of Mg2+ Glycine max
choline not Glycine max
EDTA
-
Glycine max
Mn2+ in presence of Mg2+ Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
ethanolamine pH 8.5 Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg-ATP complex is the substrate Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000 19000 gel filtration, monomeric form Glycine max
36000 37000 dimeric form, gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.73
-
pH 8.5 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ethanolamine
-
Glycine max ADP + O-phosphoethanolamine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 17000-19000, SDS-PAGE, enzyme exists in monomeric and dimeric form Glycine max
monomer 1 * 17000-19000, SDS-PAGE, gel filtration, enzyme exists in monomeric and dimeric form Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Glycine max