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Literature summary for 2.7.1.78 extracted from
- Further purification and characterization of the DNA 3-phosphatase from rat-liver chromatin which is also a polynucleotide 5-hydroxyl kinase (1988), Eur. J. Biochem., 171, 59-66.
General Stability
| General Stability | Organism |
|---|---|
| ATP protects against heat denaturation and tryptic digestion | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Rattus norvegicus |  |
| EDTA | - |
Rattus norvegicus | |
| p-hydroxymercuribenzoate | 2-mercaptoethanol prevents inhibition | Rattus norvegicus | |
| phosphate | - |
Rattus norvegicus | |
| sulfate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00069 | - |
ATP | pH 5.5, 37°C | Rattus norvegicus | |
| 0.0039 | - |
5'-OH-DNA | pH 5.5, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromatin | - |
Rattus norvegicus | 785 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cations | absolutely required | Rattus norvegicus | |
| Mg2+ | stimulates | Rattus norvegicus | |
| Mn2+ | most effective divalent cation in activation, best at 1 mM | Rattus norvegicus | |
| additional information | maximal activity at ionic strength of 135 mM | Rattus norvegicus | |
| Zn2+ | less effective | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
enzyme also has DNA 3'-phosphatase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 39fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | native and heat denatured DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + nucleoside-3'-monophosphate | - |
Rattus norvegicus | ADP + nucleoside-3',5'-diphosphate | - |
? | |
| additional information | enzyme also has DNA 3'-phosphatase activity | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme also possesses a 3'-phosphatase activity | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-optimum of 3'-phosphatase activity is 6.0 | Rattus norvegicus |
| 5.5 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Rattus norvegicus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | - |
- |
8.6 |
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Release 2023.1 (January 2023)
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