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Literature summary for 2.7.1.78 extracted from

  • Feng, C.; Wang, Z.; Chen, T.; Chen, X.; Mao, D.; Zhao, J.; Li, G.
    A dual-enzyme-assisted three-dimensional DNA walking machine using T4 polynucleotide kinase as activators and application in polynucleotide kinase assays (2018), Anal. Chem., 90, 2810-2815 .
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Tequatrovirus T4
ADP
-
 Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20 mM used in assay conditions Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
ATP + swing arm I
-
 Tequatrovirus T4 ADP + 5'-phospho-swing arm I
-
 ?

Synonyms

Synonyms Comment Organism
PNK
-
 Tequatrovirus T4
polynucleotide kinase
-
 Tequatrovirus T4

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.83
-
 at pH 8.0 and 37°C Tequatrovirus T4 ADP
7.42
-
 at pH 8.0 and 37°C Tequatrovirus T4 (NH4)2SO4