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Literature summary for 2.7.1.78 extracted from

  • Song, C.; Zhao, M.
    Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage (2009), Anal. Chem., 81, 1383-1388.
    View publication on PubMed

Application

Application Comment Organism
analysis a method for real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by use of a singly fluorophore-labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage is described Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00056
-
GGGCC(AG)10GGCCC-fluorescein
-
Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Tequatrovirus T4
-
ADP + 5'-phospho-DNA
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P06855
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (AG)10GGCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + (AG)10GGCCC-tetramethylrhodamine
-
Tequatrovirus T4 ?
-
?
ATP + 5'-dephospho-DNA
-
Tequatrovirus T4 ADP + 5'-phospho-DNA
-
?
ATP + GGGCC(AG)10GGCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + GGGCC(AG)10GGCCC-tetramethylrhodamine
-
Tequatrovirus T4 ?
-
?
ATP + GGGCC(AG)12GGCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + GGGCC(AG)8GGCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + GGGGC(AG)10GCCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + GGGGC(AG)10GCCCC-tetramethylrhodamine
-
Tequatrovirus T4 ?
-
?
ATP + GGGGG(AG)10CCCCC-fluorescein
-
Tequatrovirus T4 ?
-
?
ATP + GGGGG(AG)10CCCCC-tetramethylrhodamine
-
Tequatrovirus T4 ?
-
?

Synonyms

Synonyms Comment Organism
PNK
-
Tequatrovirus T4
polynucleotide kinase
-
Tequatrovirus T4
T4 polynucleotide kinase
-
Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
198
-
GGGCC(AG)10GGCCC-fluorescein
-
Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ATP
-
Tequatrovirus T4