Literature summary for 2.7.1.78 extracted from

Blondal, T.; Hjorleifsdottir, S.; Aevarsson, A.; Fridjonsson, O.H.; Skirnisdottir, S.; Wheat, J.O.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
Characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage RM378 (2005), J. Biol. Chem., 280, 5188-5194.

Search for more literature for 2.7.1.78

Activating Compound

Activating Compound	Comment	Organism	Structure
PEG 6000	5-15%, 3-4fold increase in activity, inhibitory above	Rhodothermus phage RM378

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	complete inhibition at 1 mM	Rhodothermus phage RM378
KCl	inhibitory above 10 mM	Rhodothermus phage RM378
Mn2+	maximum activity at 0.01 mM, inhibitory above 0.1 mM	Rhodothermus phage RM378
NaCl	inhibitory above 10 mM	Rhodothermus phage RM378
PEG 6000	5-15%, 3-4fold increase in activity, inhibitory above	Rhodothermus phage RM378

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0013	-	r(A20)	pH 6.0, 65°C	Rhodothermus phage RM378
0.0015	-	r(A20)	pH 6.0, 65°C	Rhodothermus phage RM378
0.02	-	ATP	pH 6.0, 65°C	Rhodothermus phage RM378

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	maximum activity at 0.1 mM	Rhodothermus phage RM378
Mn2+	maximum activity at 0.01 mM, inhibitory above 0.1 mM	Rhodothermus phage RM378
additional information	dovalent metal ion required	Rhodothermus phage RM378

Organism

Organism	UniProt	Comment	Textmining
Rhodothermus phage RM378	-	infecting Rhodothermus marinus, bifunctional enzyme with 5-kinase domain and 3-phosphohydrolase domain	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.67	-	substrate r(A20), pH 6.0, 65°C	Rhodothermus phage RM378
3.67	-	substrate d(A20), pH 6.0, 65°C	Rhodothermus phage RM378

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5'-dephospho-DNA	acts both on single- and blunt end double-stranded DNA	Rhodothermus phage RM378	ADP + 5'-phospho-DNA	-	?
ATP + 5'-dephospho-RNA	-	Rhodothermus phage RM378	ADP + 5'-phospho-RNA	-	?
ATP + d(A20)	-	Rhodothermus phage RM378	ADP + 5'-phospho-d(A20)	-	?
ATP + r(A20)	-	Rhodothermus phage RM378	ADP + 5'-phospho-r(A20)	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	70	-	Rhodothermus phage RM378

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	stable up to	Rhodothermus phage RM378
70	-	stable for 1 h	Rhodothermus phage RM378

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.9	-	r(A20)	pH 6.0, 65°C	Rhodothermus phage RM378
2.6	-	r(A20)	pH 6.0, 65°C	Rhodothermus phage RM378

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Rhodothermus phage RM378

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.5	10	more than 80% of maximum activity	Rhodothermus phage RM378

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Release 2023.1 (January 2023)