Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 54% remaining activity without DTT | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 50% inhibition at 8.5 mM | Bos taurus | |
diphosphate | 50% inhibition at 2.2 mM | Bos taurus | |
EDTA | complete inhibition at 25 mM | Bos taurus | |
KCl | 50% inhibition at 71 mM | Bos taurus | |
NaCl | 50% inhibition at 67 mM | Bos taurus | |
phosphate | 50% inhibition at 11 mM | Bos taurus | |
sulfate | 50% inhibition at 5.7 mM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
oligo (dT)25 | polymin P precipitable enzyme, pH 7.5, 37°C | Bos taurus | |
0.052 | - |
ATP | polymin P precipitable enzyme, pH 7.5, 37°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Bos taurus | |
Mg2+ | best at 1 mM | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
polymin P precipitable enzyme, 1570fold | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
purified, polymin P precipitable enzyme, pH 5.5, 37°C | Bos taurus |
Storage Stability | Organism |
---|---|
0°C, partially purified enzyme, 50 mM Tris-HCl, pH 7.5, 30 mM NaCl, 0.5 mM DTT, 0.5 mM EDTA, on ice, stable for several weeks | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
ATP + nucleoside-3'-monophosphate | no activity with thymidine 3'-monophosphate | Bos taurus | ADP + nucleoside-3',5'-diphosphate | - |
? | |
ATP + oligo (dT)25 | - |
Bos taurus | ? | - |
? | |
ATP + synthetic oligonucleotide | oligo (dT)25 | Bos taurus | ADP + oligonucleotide 5'-phosphate | - |
? | |
additional information | substrate specificity | Bos taurus | ? | - |
? | |
additional information | no activity with thymidine 3'-monophosphate | Bos taurus | ? | - |
? | |
additional information | no 3'-phosphatase activity | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | polymin P precipitable enzyme | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Bos taurus |