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Literature summary for 2.7.1.78 extracted from
- Purification and properties of polynucleotide kinase of calf thymus (1978), Biochim. Biophys. Acta, 522, 412-422.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | complete inhibition at 0.3 mM | Bos taurus |  |
| ammonium sulfate | 80% inhibition at 30 mM | Bos taurus | |
| CTP | more than 90% inhibition at 0.3 mM | Bos taurus | |
| dATP | more than 95% inhibition at 0.3 mM | Bos taurus | |
| dCTP | 80% inhibition at 0.3 mM | Bos taurus | |
| Dextran sulfate | - |
Bos taurus | |
| dGTP | more than 95% inhibition at 0.3 mM | Bos taurus | |
| diphosphate | 97% inhibition at 20 mM | Bos taurus | |
| dTTP | more than 95% inhibition at 0.3 mM | Bos taurus | |
| GTP | more than 90% inhibition at 0.3 mM | Bos taurus | |
| heparin | - |
Bos taurus | |
| iodoacetate | complete inhibition at 0.3 mM | Bos taurus | |
| additional information | no inhibition by agar-agar | Bos taurus | |
| sulfate | - |
Bos taurus | |
| sulfhydryl antagonists | - |
Bos taurus | |
| UTP | more than 90% inhibition at 0.3 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
ATP | pH 5.5, 37°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cations | absolutely required | Bos taurus | |
| Mg2+ | Mg2+ or Mn2+ effective in activation | Bos taurus | |
| Mn2+ | optimal concentration: 10 mM | Bos taurus | |
| Mn2+ | Mg2+ or Mn2+ effective in activation | Bos taurus | |
| Zn2+ | less effective | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration, sucrose density gradient centrifugation | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Bos taurus | - |
ADP + 5'-phospho-DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1500fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0043 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | double stranded DNA | Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | single stranded DNA | Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-RNA | phosphorylated at a much lower rate than DNA | Bos taurus | ADP + 5'-phospho-RNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000-75000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | pH 5: about 5% of activity maximum, pH 7: about 30% of activity maximum | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Bos taurus | |
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Release 2023.1 (January 2023)
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