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Literature summary for 2.7.1.78 extracted from

  • Sertic-Pritsos, K.; Vinocour, M.; Winicov, I.
    5-Hydroxyl RNA kinase from mouse L cells (1984), Eur. J. Biochem., 144, 47-55.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ inhibition above 1 mM Mus musculus
NH4+ weak inhibition Mus musculus
sulfate weak Mus musculus
Zn2+ inhibition in presence of Mg2+ Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
5'-hydroxyl poly(A) pH 8.4, 37°C Mus musculus
0.22
-
[gamma-S]ATP pH 8.4, 37°C Mus musculus
0.53
-
[gamma-S]GTP pH 8.4, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 17% of the activity with Mg2+, synergistic to Mg2+ Mus musculus
Mg2+ required Mus musculus
Mn2+ no activation, 50% inhibition at 1 mM Mus musculus
NH4+ slightly inhibitory Mus musculus
Zn2+ no activation, inhibition in presence of Mg2+ Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
1 * 93000, SDS-PAGE Mus musculus
96000
-
gel filtration, native PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 35fold Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture L-cells Mus musculus
-
L-cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0007
-
partially purified enzyme, substrate RNA Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA low activity Mus musculus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA is not effectively phosphorylated Mus musculus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-RNA much more efficient as substrate than DNA Mus musculus ADP + 5'-phospho-RNA
-
?
ATP + 5'-hydroxyl poly(A)
-
Mus musculus ?
-
?
ATP + synthetic oligonucleotide 5'-hydroxyl poly(A) Mus musculus ADP + oligonucleotide 5'-phosphate
-
?
[gamma-S]ATP + 5'-dephospho-RNA
-
Mus musculus [gamma-S]ADP + 5'-phospho-RNA
-
?
[gamma-S]GTP + 5'-dephospho-RNA
-
Mus musculus [gamma-S]GDP + 5'-phospho-RNA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 93000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
substrate DNA Mus musculus
9
-
substrate RNA Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.4 pH 7.5: about 50% of activity maximum, pH 8.4 and pH 9.4: about 85% of activity maximum Mus musculus

Cofactor

Cofactor Comment Organism Structure
4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[2-(1H-imidazol-4-yl)ethyl]piperazine-1,2-dicarboxamide
-
Mus musculus
4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[3-(1H-imidazol-4-yl)propyl]piperazine-1,2-dicarboxamide
-
Mus musculus
ATP
-
Mus musculus

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus
-
-
4.8