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Literature summary for 2.7.1.78 extracted from

  • Pheiffer, B.H.; Zimmerman, S.B.
    Deoxyribonucleic acid kinase from nuclei of rat liver: mechanism, reversal, and inhibitors of the reaction (1979), Biochemistry, 18, 2960-2963.
    View publication on PubMed
Search for more literature for 2.7.1.78

Application

Application Comment Organism
synthesis due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Dextran sulfate forward and reverse reaction at similar amounts; strong inhibition, competitive to ATP and DNA Rattus norvegicus
heparin
-
 Rattus norvegicus
additional information no inhibition by chondroitin sulfates A and C, and by dextran Rattus norvegicus
sulfate competitive to ATP, noncompetitive to DNA, less sensitive in the reverse reaction Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA random order sequential mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 r
ATP + 5'-dephospho-DNA native and denatured DNA are substrates for the reverse reaction Rattus norvegicus ADP + 5'-phospho-DNA reverse reaction is promoted by a variety of other nucleoside diphosphates but not by ATP ?
ATP + 5'-dephospho-DNA native and denatured DNA are substrates for the reverse reaction Rattus norvegicus ADP + 5'-phospho-DNA reverse reaction is promoted by a variety of other nucleoside diphosphates but not by ATP r
ATP + 5'-dephospho-DNA native and denatured DNA are substrates for the reverse reaction Rattus norvegicus ADP + 5'-phospho-DNA native and heat denatured DNA serve as substrates for the reverse reaction ?
ATP + 5'-dephospho-DNA native and denatured DNA are substrates for the reverse reaction Rattus norvegicus ADP + 5'-phospho-DNA native and heat denatured DNA serve as substrates for the reverse reaction r
ATP + 5'-dephospho-RNA no activity Rattus norvegicus ADP + 5'-phospho-RNA
-
 ?
ATP + nucleoside-3'-monophosphate no activity Rattus norvegicus ADP + nucleoside-3',5'-diphosphate
-
 ?
ATP + synthetic oligonucleotide no activity with low molecular weight oligonucleotides Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 sulfate competitive versus ATP, pH 5.5, 37°C Rattus norvegicus