Activating Compound | Comment | Organism | Structure |
---|---|---|---|
polyamines | stimulate | Homo sapiens | |
polyamines | stimulate | Rattus norvegicus | |
polyamines | stimulate | Bos taurus | |
polyamines | stimulate | Mesocricetus auratus | |
polyamines | stimulate | Tequatrovirus T4 | |
polyamines | stimulate | Escherichia phage T2 | |
polyamines | stimulate | Enterobacteria phage T6 | |
Polyethylene glycol | 4-10%, stimulates | Tequatrovirus T4 | |
spermidine | - |
Rattus norvegicus | |
spermidine | - |
Bos taurus | |
spermidine | stimulates | Tequatrovirus T4 | |
sulfhydryl reagents | - |
Homo sapiens | |
sulfhydryl reagents | essential for activity, maximal activity with 5 mM DTT, less stimulation with 2-mercaptoethanol and glutathione | Tequatrovirus T4 |
Application | Comment | Organism |
---|---|---|
synthesis | due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates | Rattus norvegicus |
synthesis | due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates | Tequatrovirus T4 |
General Stability | Organism |
---|---|
freezing and thawing reduces activity after 1 cycle by 60% | Tequatrovirus T4 |
freezing and thawing reduces activity after 1 cycle by 60% | Escherichia phage T2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Bos taurus | |
diphosphate | - |
Escherichia phage T2 | |
diphosphate | - |
Homo sapiens | |
diphosphate | - |
Rattus norvegicus | |
diphosphate | - |
Tequatrovirus T4 | |
KCl | - |
Bos taurus | |
KCl | - |
Homo sapiens | |
KCl | KCl stimulates at low concentrations, inhibits at high concentrations | Tequatrovirus T4 | |
NaCl | - |
Bos taurus | |
NaCl | - |
Homo sapiens | |
NaCl | - |
Rattus norvegicus | |
NaCl | stimulates activity towards single stranded substrates, inhibitory with some duplexes | Tequatrovirus T4 | |
NH4+ | - |
Homo sapiens | |
NH4+ | - |
Tequatrovirus T4 | |
phosphate | - |
Bos taurus | |
phosphate | - |
Escherichia phage T2 | |
phosphate | - |
Homo sapiens | |
phosphate | - |
Rattus norvegicus | |
phosphate | - |
Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Tequatrovirus T4 | |
0.004 | - |
ATP | - |
Bos taurus | |
0.004 | - |
ATP | phosphate exchange reaction between nucleotides, forward reaction, pH 7.6, 37°C | Tequatrovirus T4 | |
0.0076 | - |
5'-OH-DNA | large DNA fragments released by nuclease treatment, pH 7.6, 37°C | Tequatrovirus T4 | |
0.013 | 0.14 | ATP | depending on DNA acceptor, pH 7.6, 37°C | Tequatrovirus T4 | |
0.014 | - |
ATP | - |
Escherichia phage T2 | |
0.015 | - |
UTP | - |
Escherichia phage T2 | |
0.0222 | 0.143 | oligonucleotides | depending on the 5'-base and the length of the oligonucleotide, pH 7.6, 37°C | Tequatrovirus T4 | |
0.025 | - |
CTP | - |
Escherichia phage T2 | |
0.033 | - |
GTP | - |
Escherichia phage T2 | |
0.2 | - |
ADP | dephosphorylation of single-stranded oligonucleotides, pH 7.6, 37°C | Tequatrovirus T4 | |
0.2 | - |
ADP | phosphate exchange reaction between nucleotides, reverse reaction, pH 7.6, 37°C | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Rattus norvegicus | |
K+ | - |
Bos taurus | |
K+ | KCl stimulates at low concentrations, inhibits at high concentrations | Tequatrovirus T4 | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Escherichia phage T2 | |
Mg2+ | required | Bos taurus | |
Mg2+ | required | Tequatrovirus T4 | |
Mg2+ | optimal concentration is 10 mM | Bos taurus | |
Mg2+ | optimal concentration is 10 mM | Tequatrovirus T4 | |
Mg2+ | optimal concentration at pH optimum 7.6 is 10 mM | Tequatrovirus T4 | |
Mn2+ | - |
Bos taurus | |
Mn2+ | - |
Escherichia phage T2 | |
Mn2+ | can partially replace Mg2+ in activation | Homo sapiens | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Tequatrovirus T4 | |
Mn2+ | at 3.3 mM 50% of the activity with 10 mM Mg2+ | Tequatrovirus T4 | |
Na+ | - |
Rattus norvegicus | |
Na+ | stimulates activity towards single stranded substrates, inhibitory to some duplexes | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Tequatrovirus T4 |
33200 | - |
4 * 33200, analytical ultracentrifugation | Tequatrovirus T4 |
80000 | - |
gel filtration | Rattus norvegicus |
140000 | - |
gel filtration | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Homo sapiens | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | Bos taurus | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | Escherichia phage T2 | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Enterobacteria phage T6 | - |
ADP + 5'-phospho-DNA | - |
? | |
additional information | Tequatrovirus T4 | in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Enterobacteria phage T6 | - |
- |
- |
Escherichia phage T2 | - |
from infected Escherichia coli | - |
Homo sapiens | - |
HeLa cells | - |
Mesocricetus auratus | - |
chinese hamster lung cells | - |
no activity in Escherichia coli | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Tequatrovirus T4 | - |
from infected Escherichia coli | - |
Tequatrovirus T4 | - |
gene pseT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | HeLa cells | Homo sapiens | - |
cell culture | chinese hamster lung cells | Mesocricetus auratus | - |
HeLa cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | - |
Mesocricetus auratus | - |
lung | - |
Mesocricetus auratus | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | 40 | commercial preparation | Tequatrovirus T4 |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 7.5, 25 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.001 mM ATP, 50% glycerol, stable for up to 18 months | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Homo sapiens | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | - |
Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Mesocricetus auratus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | - |
Escherichia phage T2 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Enterobacteria phage T6 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | double stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | double stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | double stranded DNA | Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | double stranded DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | ? | |
ATP + 5'-dephospho-DNA | double stranded DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | r | |
ATP + 5'-dephospho-DNA | large DNA fragments released by nuclease treatment | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | large DNA fragments released by nuclease treatment | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | large DNA fragments released by nuclease treatment | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | ? | |
ATP + 5'-dephospho-DNA | large DNA fragments released by nuclease treatment | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | r | |
ATP + 5'-dephospho-DNA | single stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | single stranded DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-DNA | single stranded DNA | Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | single stranded DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | ? | |
ATP + 5'-dephospho-DNA | single stranded DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | excess ADP will cause the reverse reaction to be favored | r | |
ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Escherichia phage T2 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Enterobacteria phage T6 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | no activity | Homo sapiens | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | no activity | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | no activity | Bos taurus | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | no activity | Mesocricetus auratus | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | low activity | Bos taurus | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | enzyme cannot act on RNA less than 10 bases in length | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | solely RNA-specific | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
ATP + deoxynucleoside 3'-phosphate | - |
Tequatrovirus T4 | ADP + deoxynucleoside 3',5'-diphosphate | - |
? | |
ATP + nucleoside-3'-monophosphate | - |
Rattus norvegicus | ADP + nucleoside-3',5'-diphosphate | - |
? | |
ATP + nucleoside-3'-monophosphate | - |
Tequatrovirus T4 | ADP + nucleoside-3',5'-diphosphate | - |
? | |
ATP + nucleoside-3'-monophosphate | - |
Escherichia phage T2 | ADP + nucleoside-3',5'-diphosphate | - |
? | |
ATP + oligonucleotides | - |
Tequatrovirus T4 | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | - |
Homo sapiens | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | - |
Bos taurus | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | - |
Tequatrovirus T4 | ADP + oligonucleotide 5'-phosphate | - |
? | |
ATP + synthetic oligonucleotide | oligodeoxynucleotides of chain length above 10-12 residues | Rattus norvegicus | ADP + oligonucleotide 5'-phosphate | - |
? | |
CTP + 5'-dephospho-DNA | - |
Homo sapiens | CDP + 5'-phospho-DNA | - |
? | |
CTP + 5'-dephospho-DNA | - |
Rattus norvegicus | CDP + 5'-phospho-DNA | - |
? | |
CTP + 5'-dephospho-DNA | - |
Bos taurus | CDP + 5'-phospho-DNA | - |
? | |
CTP + 5'-dephospho-DNA | - |
Mesocricetus auratus | CDP + 5'-phospho-DNA | - |
? | |
CTP + 5'-dephospho-DNA | - |
Escherichia phage T2 | CDP + 5'-phospho-DNA | - |
? | |
dATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | dADP + 5'-phospho-DNA | - |
? | |
GTP + 5'-dephospho-DNA | - |
Homo sapiens | GDP + 5'-phospho-DNA | - |
? | |
GTP + 5'-dephospho-DNA | - |
Rattus norvegicus | GDP + 5'-phospho-DNA | - |
? | |
GTP + 5'-dephospho-DNA | - |
Bos taurus | GDP + 5'-phospho-DNA | - |
? | |
GTP + 5'-dephospho-DNA | - |
Mesocricetus auratus | GDP + 5'-phospho-DNA | - |
? | |
GTP + 5'-dephospho-DNA | - |
Escherichia phage T2 | GDP + 5'-phospho-DNA | - |
? | |
additional information | - |
Escherichia phage T2 | ? | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Homo sapiens | ? | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Rattus norvegicus | ? | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Bos taurus | ? | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Tequatrovirus T4 | ? | - |
? | |
additional information | in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging | Tequatrovirus T4 | ? | - |
? | |
TTP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | TDP + 5'-phospho-DNA | - |
? | |
UTP + 5'-dephospho-DNA | - |
Rattus norvegicus | UDP + 5'-phospho-DNA | - |
? | |
UTP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | UDP + 5'-phospho-DNA | - |
? | |
UTP + 5'-dephospho-DNA | - |
Escherichia phage T2 | UDP + 5'-phospho-DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Tequatrovirus T4 |
tetramer | 4 * 33200, analytical ultracentrifugation | Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme also possesses a 3'-phosphatase activity | Homo sapiens |
More | enzyme also possesses a 3'-phosphatase activity | Rattus norvegicus |
More | enzyme also possesses a 3'-phosphatase activity | Bos taurus |
More | enzyme also possesses a 3'-phosphatase activity | Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
37 | - |
- |
Rattus norvegicus |
37 | - |
- |
Bos taurus |
37 | - |
- |
Tequatrovirus T4 |
37 | - |
- |
Escherichia phage T2 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
37 | 0°C: about 7% of activity maximum, 37°C: activity maximum, bacteriophage T4 enzyme | Tequatrovirus T4 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
marked temperature lability in absence of substrate | Homo sapiens |
25 | - |
marked temperature lability in absence of substrate | Rattus norvegicus |
25 | - |
marked temperature lability in absence of substrate | Bos taurus |
25 | - |
marked temperature lability in absence of substrate | Mesocricetus auratus |
25 | - |
marked temperature lability in absence of substrate | Tequatrovirus T4 |
25 | - |
marked temperature lability in absence of substrate | Escherichia phage T2 |
25 | - |
marked temperature lability in absence of substrate | Enterobacteria phage T6 |
37 | - |
- |
Escherichia phage T2 |
37 | - |
15 min, 77% loss of activity | Tequatrovirus T4 |
45 | - |
- |
Homo sapiens |
45 | - |
5 min, 92% loss of activity | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
imidazole buffer | Tequatrovirus T4 |
7.6 | - |
Tris buffer | Tequatrovirus T4 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | forward reaction, optimal range | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | reverse reaction | Tequatrovirus T4 | |
ATP | dependent on | Homo sapiens | |
ATP | dependent on | Rattus norvegicus | |
ATP | dependent on | Bos taurus | |
ATP | dependent on | Mesocricetus auratus | |
ATP | dependent on | Tequatrovirus T4 | |
ATP | dependent on | Escherichia phage T2 | |
ATP | dependent on | Enterobacteria phage T6 | |
CDP | - |
Homo sapiens | |
CDP | - |
Rattus norvegicus | |
CDP | - |
Bos taurus | |
CDP | - |
Mesocricetus auratus | |
CDP | - |
Escherichia phage T2 | |
CTP | - |
Homo sapiens | |
CTP | - |
Rattus norvegicus | |
CTP | - |
Bos taurus | |
CTP | - |
Mesocricetus auratus | |
CTP | - |
Escherichia phage T2 | |
dATP | - |
Tequatrovirus T4 | |
GDP | - |
Homo sapiens | |
GDP | - |
Rattus norvegicus | |
GDP | - |
Bos taurus | |
GDP | - |
Mesocricetus auratus | |
GDP | - |
Escherichia phage T2 | |
GTP | - |
Homo sapiens | |
GTP | - |
Rattus norvegicus | |
GTP | - |
Bos taurus | |
GTP | - |
Mesocricetus auratus | |
GTP | - |
Escherichia phage T2 | |
TDP | - |
Tequatrovirus T4 | |
TTP | - |
Tequatrovirus T4 | |
UDP | - |
Rattus norvegicus | |
UDP | - |
Tequatrovirus T4 | |
UDP | - |
Escherichia phage T2 | |
UTP | - |
Rattus norvegicus | |
UTP | - |
Tequatrovirus T4 | |
UTP | - |
Escherichia phage T2 |