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Literature summary for 2.7.1.78 extracted from

  • Maunders, M.J.
    Polynucleotide kinase (EC 2.7.1.78) (1993), Methods Mol. Biol., 16, 343-357.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
polyamines stimulate Homo sapiens
polyamines stimulate Rattus norvegicus
polyamines stimulate Bos taurus
polyamines stimulate Mesocricetus auratus
polyamines stimulate Tequatrovirus T4
polyamines stimulate Escherichia phage T2
polyamines stimulate Enterobacteria phage T6
Polyethylene glycol 4-10%, stimulates Tequatrovirus T4
spermidine
-
Rattus norvegicus
spermidine
-
Bos taurus
spermidine stimulates Tequatrovirus T4
sulfhydryl reagents
-
Homo sapiens
sulfhydryl reagents essential for activity, maximal activity with 5 mM DTT, less stimulation with 2-mercaptoethanol and glutathione Tequatrovirus T4

Application

Application Comment Organism
synthesis due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates Rattus norvegicus
synthesis due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates Tequatrovirus T4

General Stability

General Stability Organism
freezing and thawing reduces activity after 1 cycle by 60% Tequatrovirus T4
freezing and thawing reduces activity after 1 cycle by 60% Escherichia phage T2

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
Bos taurus
diphosphate
-
Escherichia phage T2
diphosphate
-
Homo sapiens
diphosphate
-
Rattus norvegicus
diphosphate
-
Tequatrovirus T4
KCl
-
Bos taurus
KCl
-
Homo sapiens
KCl KCl stimulates at low concentrations, inhibits at high concentrations Tequatrovirus T4
NaCl
-
Bos taurus
NaCl
-
Homo sapiens
NaCl
-
Rattus norvegicus
NaCl stimulates activity towards single stranded substrates, inhibitory with some duplexes Tequatrovirus T4
NH4+
-
Homo sapiens
NH4+
-
Tequatrovirus T4
phosphate
-
Bos taurus
phosphate
-
Escherichia phage T2
phosphate
-
Homo sapiens
phosphate
-
Rattus norvegicus
phosphate
-
Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Tequatrovirus T4
0.004
-
ATP
-
Bos taurus
0.004
-
ATP phosphate exchange reaction between nucleotides, forward reaction, pH 7.6, 37°C Tequatrovirus T4
0.0076
-
5'-OH-DNA large DNA fragments released by nuclease treatment, pH 7.6, 37°C Tequatrovirus T4
0.013 0.14 ATP depending on DNA acceptor, pH 7.6, 37°C Tequatrovirus T4
0.014
-
ATP
-
Escherichia phage T2
0.015
-
UTP
-
Escherichia phage T2
0.0222 0.143 oligonucleotides depending on the 5'-base and the length of the oligonucleotide, pH 7.6, 37°C Tequatrovirus T4
0.025
-
CTP
-
Escherichia phage T2
0.033
-
GTP
-
Escherichia phage T2
0.2
-
ADP dephosphorylation of single-stranded oligonucleotides, pH 7.6, 37°C Tequatrovirus T4
0.2
-
ADP phosphate exchange reaction between nucleotides, reverse reaction, pH 7.6, 37°C Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Rattus norvegicus
K+
-
Bos taurus
K+ KCl stimulates at low concentrations, inhibits at high concentrations Tequatrovirus T4
Mg2+
-
Homo sapiens
Mg2+
-
Rattus norvegicus
Mg2+
-
Escherichia phage T2
Mg2+ required Bos taurus
Mg2+ required Tequatrovirus T4
Mg2+ optimal concentration is 10 mM Bos taurus
Mg2+ optimal concentration is 10 mM Tequatrovirus T4
Mg2+ optimal concentration at pH optimum 7.6 is 10 mM Tequatrovirus T4
Mn2+
-
Bos taurus
Mn2+
-
Escherichia phage T2
Mn2+ can partially replace Mg2+ in activation Homo sapiens
Mn2+ can partially replace Mg2+ in activation Rattus norvegicus
Mn2+ can partially replace Mg2+ in activation Tequatrovirus T4
Mn2+ at 3.3 mM 50% of the activity with 10 mM Mg2+ Tequatrovirus T4
Na+
-
Rattus norvegicus
Na+ stimulates activity towards single stranded substrates, inhibitory to some duplexes Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Tequatrovirus T4
33200
-
4 * 33200, analytical ultracentrifugation Tequatrovirus T4
80000
-
gel filtration Rattus norvegicus
140000
-
gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Homo sapiens
-
ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA Rattus norvegicus
-
ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA Rattus norvegicus
-
ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA Bos taurus
-
ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA Tequatrovirus T4
-
ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA Escherichia phage T2
-
ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA Enterobacteria phage T6
-
ADP + 5'-phospho-DNA
-
?
additional information Tequatrovirus T4 in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Enterobacteria phage T6
-
-
-
Escherichia phage T2
-
from infected Escherichia coli
-
Homo sapiens
-
HeLa cells
-
Mesocricetus auratus
-
chinese hamster lung cells
-
no activity in Escherichia coli
-
-
-
Rattus norvegicus
-
-
-
Tequatrovirus T4
-
from infected Escherichia coli
-
Tequatrovirus T4
-
gene pseT
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture HeLa cells Homo sapiens
-
cell culture chinese hamster lung cells Mesocricetus auratus
-
HeLa cell
-
Homo sapiens
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-
liver
-
Mesocricetus auratus
-
lung
-
Mesocricetus auratus
-
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30 40 commercial preparation Tequatrovirus T4

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.5, 25 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.001 mM ATP, 50% glycerol, stable for up to 18 months Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
Homo sapiens ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA
-
Rattus norvegicus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA
-
Rattus norvegicus ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA
-
Bos taurus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA
-
Mesocricetus auratus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA
-
Tequatrovirus T4 ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA
-
Escherichia phage T2 ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA
-
Enterobacteria phage T6 ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA double stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA double stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA double stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA double stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
ATP + 5'-dephospho-DNA double stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Rattus norvegicus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Rattus norvegicus ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
r
ATP + 5'-dephospho-DNA single stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
?
ATP + 5'-dephospho-DNA single stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
ATP + 5'-dephospho-DNA single stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
ATP + 5'-dephospho-RNA
-
Tequatrovirus T4 ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA
-
Escherichia phage T2 ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA
-
Enterobacteria phage T6 ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA no activity Homo sapiens ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA no activity Rattus norvegicus ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA no activity Bos taurus ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA no activity Mesocricetus auratus ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA low activity Bos taurus ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA enzyme cannot act on RNA less than 10 bases in length Rattus norvegicus ADP + 5'-phospho-RNA
-
?
ATP + 5'-dephospho-RNA solely RNA-specific Rattus norvegicus ADP + 5'-phospho-RNA
-
?
ATP + deoxynucleoside 3'-phosphate
-
Tequatrovirus T4 ADP + deoxynucleoside 3',5'-diphosphate
-
?
ATP + nucleoside-3'-monophosphate
-
Rattus norvegicus ADP + nucleoside-3',5'-diphosphate
-
?
ATP + nucleoside-3'-monophosphate
-
Tequatrovirus T4 ADP + nucleoside-3',5'-diphosphate
-
?
ATP + nucleoside-3'-monophosphate
-
Escherichia phage T2 ADP + nucleoside-3',5'-diphosphate
-
?
ATP + oligonucleotides
-
Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
?
ATP + synthetic oligonucleotide
-
Homo sapiens ADP + oligonucleotide 5'-phosphate
-
?
ATP + synthetic oligonucleotide
-
Bos taurus ADP + oligonucleotide 5'-phosphate
-
?
ATP + synthetic oligonucleotide
-
Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
?
ATP + synthetic oligonucleotide oligodeoxynucleotides of chain length above 10-12 residues Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
?
CTP + 5'-dephospho-DNA
-
Homo sapiens CDP + 5'-phospho-DNA
-
?
CTP + 5'-dephospho-DNA
-
Rattus norvegicus CDP + 5'-phospho-DNA
-
?
CTP + 5'-dephospho-DNA
-
Bos taurus CDP + 5'-phospho-DNA
-
?
CTP + 5'-dephospho-DNA
-
Mesocricetus auratus CDP + 5'-phospho-DNA
-
?
CTP + 5'-dephospho-DNA
-
Escherichia phage T2 CDP + 5'-phospho-DNA
-
?
dATP + 5'-dephospho-DNA
-
Tequatrovirus T4 dADP + 5'-phospho-DNA
-
?
GTP + 5'-dephospho-DNA
-
Homo sapiens GDP + 5'-phospho-DNA
-
?
GTP + 5'-dephospho-DNA
-
Rattus norvegicus GDP + 5'-phospho-DNA
-
?
GTP + 5'-dephospho-DNA
-
Bos taurus GDP + 5'-phospho-DNA
-
?
GTP + 5'-dephospho-DNA
-
Mesocricetus auratus GDP + 5'-phospho-DNA
-
?
GTP + 5'-dephospho-DNA
-
Escherichia phage T2 GDP + 5'-phospho-DNA
-
?
additional information
-
Escherichia phage T2 ?
-
?
additional information enzyme also has DNA 3'-phosphatase activity Homo sapiens ?
-
?
additional information enzyme also has DNA 3'-phosphatase activity Rattus norvegicus ?
-
?
additional information enzyme also has DNA 3'-phosphatase activity Bos taurus ?
-
?
additional information enzyme also has DNA 3'-phosphatase activity Tequatrovirus T4 ?
-
?
additional information in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging Tequatrovirus T4 ?
-
?
TTP + 5'-dephospho-DNA
-
Tequatrovirus T4 TDP + 5'-phospho-DNA
-
?
UTP + 5'-dephospho-DNA
-
Rattus norvegicus UDP + 5'-phospho-DNA
-
?
UTP + 5'-dephospho-DNA
-
Tequatrovirus T4 UDP + 5'-phospho-DNA
-
?
UTP + 5'-dephospho-DNA
-
Escherichia phage T2 UDP + 5'-phospho-DNA
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Tequatrovirus T4
tetramer 4 * 33200, analytical ultracentrifugation Tequatrovirus T4

Synonyms

Synonyms Comment Organism
More enzyme also possesses a 3'-phosphatase activity Homo sapiens
More enzyme also possesses a 3'-phosphatase activity Rattus norvegicus
More enzyme also possesses a 3'-phosphatase activity Bos taurus
More enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Homo sapiens
37
-
-
Rattus norvegicus
37
-
-
Bos taurus
37
-
-
Tequatrovirus T4
37
-
-
Escherichia phage T2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
37 0°C: about 7% of activity maximum, 37°C: activity maximum, bacteriophage T4 enzyme Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
marked temperature lability in absence of substrate Homo sapiens
25
-
marked temperature lability in absence of substrate Rattus norvegicus
25
-
marked temperature lability in absence of substrate Bos taurus
25
-
marked temperature lability in absence of substrate Mesocricetus auratus
25
-
marked temperature lability in absence of substrate Tequatrovirus T4
25
-
marked temperature lability in absence of substrate Escherichia phage T2
25
-
marked temperature lability in absence of substrate Enterobacteria phage T6
37
-
-
Escherichia phage T2
37
-
15 min, 77% loss of activity Tequatrovirus T4
45
-
-
Homo sapiens
45
-
5 min, 92% loss of activity Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
imidazole buffer Tequatrovirus T4
7.6
-
Tris buffer Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8 forward reaction, optimal range Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ADP reverse reaction Tequatrovirus T4
ATP dependent on Homo sapiens
ATP dependent on Rattus norvegicus
ATP dependent on Bos taurus
ATP dependent on Mesocricetus auratus
ATP dependent on Tequatrovirus T4
ATP dependent on Escherichia phage T2
ATP dependent on Enterobacteria phage T6
CDP
-
Homo sapiens
CDP
-
Rattus norvegicus
CDP
-
Bos taurus
CDP
-
Mesocricetus auratus
CDP
-
Escherichia phage T2
CTP
-
Homo sapiens
CTP
-
Rattus norvegicus
CTP
-
Bos taurus
CTP
-
Mesocricetus auratus
CTP
-
Escherichia phage T2
dATP
-
Tequatrovirus T4
GDP
-
Homo sapiens
GDP
-
Rattus norvegicus
GDP
-
Bos taurus
GDP
-
Mesocricetus auratus
GDP
-
Escherichia phage T2
GTP
-
Homo sapiens
GTP
-
Rattus norvegicus
GTP
-
Bos taurus
GTP
-
Mesocricetus auratus
GTP
-
Escherichia phage T2
TDP
-
Tequatrovirus T4
TTP
-
Tequatrovirus T4
UDP
-
Rattus norvegicus
UDP
-
Tequatrovirus T4
UDP
-
Escherichia phage T2
UTP
-
Rattus norvegicus
UTP
-
Tequatrovirus T4
UTP
-
Escherichia phage T2