BRENDA - Enzyme Database show
show all sequences of 2.7.1.77

Characterization of nucleoside phosphotransferase from Leishmania tropica

Shin, I.S.; Wataya, Y.; Nucleosides Nucleotides 14, 13-22 (1995)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0057
-
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
-
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
-
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0061
-
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0063
-
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0064
-
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0066
-
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0071
-
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0084
-
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.014
-
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0153
-
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0167
-
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0169
-
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Leishmania tropica
5737
-
membrane
-
Leishmania tropica
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Leishmania tropica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leishmania tropica
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Leishmania tropica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxycytidine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-fluoroinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
-
-
-
?
4-nitrophenyl phosphate + adenosine
-
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
-
-
-
?
4-nitrophenyl phosphate + cytidine
-
642003
Leishmania tropica
4-nitrophenol + CMP
-
-
-
?
4-nitrophenyl phosphate + guanosine
-
642003
Leishmania tropica
4-nitrophenol + GMP
-
-
-
?
4-nitrophenyl phosphate + inosine
-
642003
Leishmania tropica
4-nitrophenol + IMP
-
-
-
?
4-nitrophenyl phosphate + thymidine
-
642003
Leishmania tropica
4-nitrophenol + TMP
-
-
-
?
4-nitrophenyl phosphate + uridine
-
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0057
-
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
-
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
-
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0061
-
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0063
-
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0064
-
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0066
-
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0071
-
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0084
-
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.014
-
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0153
-
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0167
-
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0169
-
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Leishmania tropica
5737
-
membrane
-
Leishmania tropica
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Leishmania tropica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Leishmania tropica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxycytidine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
?
4-nitrophenyl phosphate + 3'-fluoroinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
-
-
-
?
4-nitrophenyl phosphate + adenosine
-
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
-
-
-
?
4-nitrophenyl phosphate + cytidine
-
642003
Leishmania tropica
4-nitrophenol + CMP
-
-
-
?
4-nitrophenyl phosphate + guanosine
-
642003
Leishmania tropica
4-nitrophenol + GMP
-
-
-
?
4-nitrophenyl phosphate + inosine
-
642003
Leishmania tropica
4-nitrophenol + IMP
-
-
-
?
4-nitrophenyl phosphate + thymidine
-
642003
Leishmania tropica
4-nitrophenol + TMP
-
-
-
?
4-nitrophenyl phosphate + uridine
-
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
-
-
-
?
Other publictions for EC 2.7.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-