Data extracted from this reference:
KM Value [mM]
0.0057
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0061
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0063
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0064
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0066
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0071
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0084
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.014
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0153
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0167
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0169
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization
cytoplasm
Leishmania tropica
5737
membrane
Leishmania tropica
16020
Molecular Weight [Da]
40000
gel filtration
Leishmania tropica
Organism
Specific Activity [micromol/min/mg]
Substrates and Products (Substrate)
4-nitrophenyl phosphate + 2'-deoxyadenosine
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
?
4-nitrophenyl phosphate + 2'-deoxycytidine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
?
4-nitrophenyl phosphate + 2'-deoxyinosine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-deoxyinosine
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-fluoroinosine
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
?
4-nitrophenyl phosphate + adenosine
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
?
4-nitrophenyl phosphate + cytidine
642003
Leishmania tropica
4-nitrophenol + CMP
?
4-nitrophenyl phosphate + guanosine
642003
Leishmania tropica
4-nitrophenol + GMP
?
4-nitrophenyl phosphate + inosine
642003
Leishmania tropica
4-nitrophenol + IMP
?
4-nitrophenyl phosphate + thymidine
642003
Leishmania tropica
4-nitrophenol + TMP
?
4-nitrophenyl phosphate + uridine
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
?
KM Value [mM] (protein specific)
0.0057
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0059
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0061
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0063
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0064
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0066
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0071
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0084
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.014
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0153
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0167
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
0.0169
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization (protein specific)
cytoplasm
Leishmania tropica
5737
membrane
Leishmania tropica
16020
Molecular Weight [Da] (protein specific)
40000
gel filtration
Leishmania tropica
Specific Activity [micromol/min/mg] (protein specific)
Substrates and Products (Substrate) (protein specific)
4-nitrophenyl phosphate + 2'-deoxyadenosine
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
?
4-nitrophenyl phosphate + 2'-deoxycytidine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
?
4-nitrophenyl phosphate + 2'-deoxyinosine
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-deoxyinosine
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
?
4-nitrophenyl phosphate + 3'-fluoroinosine
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
?
4-nitrophenyl phosphate + adenosine
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
?
4-nitrophenyl phosphate + cytidine
642003
Leishmania tropica
4-nitrophenol + CMP
?
4-nitrophenyl phosphate + guanosine
642003
Leishmania tropica
4-nitrophenol + GMP
?
4-nitrophenyl phosphate + inosine
642003
Leishmania tropica
4-nitrophenol + IMP
?
4-nitrophenyl phosphate + thymidine
642003
Leishmania tropica
4-nitrophenol + TMP
?
4-nitrophenyl phosphate + uridine
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
?
Other publictions for EC 2.7.1.77
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
-
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
1
-
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-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-