BRENDA - Enzyme Database show
show all sequences of 2.7.1.77

Nucleoside phosphotransferase from carrot

Brunngraber, E.F.; Methods Enzymol. 51, 387-394 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NaF
0.25 M, enhances activity by about 45%
Daucus carota
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Daucus carota
cysteine
-
Daucus carota
EDTA
-
Daucus carota
Fe2+
-
Daucus carota
Fe3+
-
Daucus carota
glutathione
-
Daucus carota
Iodine
-
Daucus carota
Mn2+
-
Daucus carota
NaF
0.25 M, phosphatase is inhibited by less than 10%
Daucus carota
Pb2+
-
Daucus carota
uridine
inhibitor of phosphatase reaction
Daucus carota
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
Phenylphosphate
phosphotransferase reaction with uridine
Daucus carota
3.5
-
uridine
phosphotransferase reaction with phenyl phosphate
Daucus carota
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation
Daucus carota
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Daucus carota
-
-
-
Purification (Commentary)
Commentary
Organism
-
Daucus carota
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
-
Daucus carota
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 1 months
Daucus carota
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
-
641991
Daucus carota
?
-
-
-
-
phenyl phosphate + adenosine
-
641991
Daucus carota
AMP + phenol
-
-
-
?
phenyl phosphate + cytidine
-
641991
Daucus carota
CMP + phenol
-
-
-
?
phenyl phosphate + deoxyadenosine
-
641991
Daucus carota
dAMP + phenol
-
-
-
?
phenyl phosphate + deoxycytidine
-
641991
Daucus carota
dCMP + phenol
-
-
-
?
phenyl phosphate + deoxyguanosine
-
641991
Daucus carota
dGMP + phenol
-
-
-
?
phenyl phosphate + deoxyuridine
-
641991
Daucus carota
dUMP + phenol
-
-
-
?
phenyl phosphate + guanosine
-
641991
Daucus carota
GMP + phenol
-
-
-
?
phenyl phosphate + thymidine
-
641991
Daucus carota
TMP + phenol
-
-
-
?
phenyl phosphate + uridine
-
641991
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Daucus carota
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
9.5
-
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h
Daucus carota
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Daucus carota
intact enzyme
-
4.1
Daucus carota
subunit A
4.7
4.6
Daucus carota
subunit B
-
5.1
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NaF
0.25 M, enhances activity by about 45%
Daucus carota
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Daucus carota
cysteine
-
Daucus carota
EDTA
-
Daucus carota
Fe2+
-
Daucus carota
Fe3+
-
Daucus carota
glutathione
-
Daucus carota
Iodine
-
Daucus carota
Mn2+
-
Daucus carota
NaF
0.25 M, phosphatase is inhibited by less than 10%
Daucus carota
Pb2+
-
Daucus carota
uridine
inhibitor of phosphatase reaction
Daucus carota
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
Phenylphosphate
phosphotransferase reaction with uridine
Daucus carota
3.5
-
uridine
phosphotransferase reaction with phenyl phosphate
Daucus carota
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation
Daucus carota
Purification (Commentary) (protein specific)
Commentary
Organism
-
Daucus carota
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
-
Daucus carota
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 1 months
Daucus carota
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
-
641991
Daucus carota
?
-
-
-
-
phenyl phosphate + adenosine
-
641991
Daucus carota
AMP + phenol
-
-
-
?
phenyl phosphate + cytidine
-
641991
Daucus carota
CMP + phenol
-
-
-
?
phenyl phosphate + deoxyadenosine
-
641991
Daucus carota
dAMP + phenol
-
-
-
?
phenyl phosphate + deoxycytidine
-
641991
Daucus carota
dCMP + phenol
-
-
-
?
phenyl phosphate + deoxyguanosine
-
641991
Daucus carota
dGMP + phenol
-
-
-
?
phenyl phosphate + deoxyuridine
-
641991
Daucus carota
dUMP + phenol
-
-
-
?
phenyl phosphate + guanosine
-
641991
Daucus carota
GMP + phenol
-
-
-
?
phenyl phosphate + thymidine
-
641991
Daucus carota
TMP + phenol
-
-
-
?
phenyl phosphate + uridine
-
641991
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Daucus carota
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
9.5
-
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h
Daucus carota
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Daucus carota
intact enzyme
-
4.1
Daucus carota
subunit A
4.7
4.6
Daucus carota
subunit B
-
5.1
Other publictions for EC 2.7.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-