BRENDA - Enzyme Database show
show all sequences of 2.7.1.77

Purification and properties of a nucleoside phosphotransferase from carrot

Brunngraber, E.F.; Chargaff, E.; J. Biol. Chem. 242, 4834-4840 (1967)

Data extracted from this reference:

General Stability
General Stability
Organism
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Daucus carota
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
Fe2+
-
Daucus carota
Fe3+
-
Daucus carota
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
Mn2+
-
Daucus carota
Pb2+
-
Daucus carota
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Daucus carota
-
-
-
Purification (Commentary)
Commentary
Organism
-
Daucus carota
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Daucus carota
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 1 months
Daucus carota
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
-
-
-
?
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
-
-
-
?
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
-
-
-
?
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
-
-
-
-
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
-
-
-
-
phenyl phosphate + adenosine
-
641990
Daucus carota
AMP + phenol
-
-
-
?
phenyl phosphate + cytidine
-
641990
Daucus carota
CMP + phenol
-
-
-
?
phenyl phosphate + deoxyadenosine
-
641990
Daucus carota
dAMP + phenol
-
-
-
?
phenyl phosphate + deoxycytidine
-
641990
Daucus carota
dCMP + phenol
-
-
-
?
phenyl phosphate + deoxyguanosine
-
641990
Daucus carota
dGMP + phenol
-
-
-
?
phenyl phosphate + deoxyuridine
-
641990
Daucus carota
dUMP + phenol
-
-
-
?
phenyl phosphate + guanosine
-
641990
Daucus carota
GMP + phenol
-
-
-
?
phenyl phosphate + thymidine
-
641990
Daucus carota
TMP + phenol
-
-
-
?
phenyl phosphate + uridine
-
641990
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota
General Stability (protein specific)
General Stability
Organism
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
Daucus carota
no loss of activity after dialysis overnight against distilled water
Daucus carota
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Daucus carota
cysteine
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
EDTA
0.625 mM, inhibition is reversed by 5 mM MgCl2
Daucus carota
Fe2+
-
Daucus carota
Fe3+
-
Daucus carota
glutathione
2.5 mM, depresses both transferase and phosphatase activity by about 25%
Daucus carota
Mn2+
-
Daucus carota
Pb2+
-
Daucus carota
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Cu2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Mg2+
enhances phosphatase activity considerably, but transferase activity to a small extent
Daucus carota
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed
Daucus carota
Purification (Commentary) (protein specific)
Commentary
Organism
-
Daucus carota
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Daucus carota
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Daucus carota
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 1 months
Daucus carota
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
Daucus carota
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2',3')-uridylic acid + uridine
about 10% of the activity with phenyl phosphate
641990
Daucus carota
uridine + UMP
-
-
-
?
3'-AMP + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
5'-adenylic acid + uridine
70% of the activity with phenyl phosphate
641990
Daucus carota
adenosine + UMP
-
-
-
?
beta-glycerophosphate + uridine
about 15% of the activity with phenyl phosphate
641990
Daucus carota
glycerol + UMP
-
-
-
?
D-ribose 5-phosphate + uridine
about 30% of the activity with phenyl phosphate
641990
Daucus carota
D-ribose + UMP
-
-
-
?
additional information
the enzyme also exhibits phosphatase activity
641990
Daucus carota
?
-
-
-
-
additional information
protein carries both transfer and hydrolytic functions
641990
Daucus carota
?
-
-
-
-
phenyl phosphate + adenosine
-
641990
Daucus carota
AMP + phenol
-
-
-
?
phenyl phosphate + cytidine
-
641990
Daucus carota
CMP + phenol
-
-
-
?
phenyl phosphate + deoxyadenosine
-
641990
Daucus carota
dAMP + phenol
-
-
-
?
phenyl phosphate + deoxycytidine
-
641990
Daucus carota
dCMP + phenol
-
-
-
?
phenyl phosphate + deoxyguanosine
-
641990
Daucus carota
dGMP + phenol
-
-
-
?
phenyl phosphate + deoxyuridine
-
641990
Daucus carota
dUMP + phenol
-
-
-
?
phenyl phosphate + guanosine
-
641990
Daucus carota
GMP + phenol
-
-
-
?
phenyl phosphate + thymidine
-
641990
Daucus carota
TMP + phenol
-
-
-
?
phenyl phosphate + uridine
-
641990
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
reaction with phenylphosphate and uridine or deoxycytidine
Daucus carota
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
7
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
Daucus carota
Other publictions for EC 2.7.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672755
Birichevskaya
A comparison of enzymatic phos ...
Pantoea agglomerans
Biotechnol. Lett.
29
585-591
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
1
-
-
7
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658328
Barai
An improved method for the enz ...
Pantoea agglomerans
Biotechnol. Lett.
26
1847-1850
2004
-
1
-
-
-
-
-
-
-
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2
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-
-
-
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-
-
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-
-
1
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642002
Asano
A novel selective nucleoside p ...
Morganella morganii, Morganella morganii NCIMB10466
J. Biosci. Bioeng.
87
732-738
1999
-
-
-
1
-
-
7
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
7
-
2
-
-
2
-
-
-
-
1
-
-
1
-
21
1
1
-
2
-
2
1
1
-
-
-
-
-
-
-
642003
Shin
-
Characterization of nucleoside ...
Leishmania tropica
Nucleosides Nucleotides
14
13-22
1995
-
-
-
-
-
-
-
13
2
-
1
-
-
1
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
2
-
1
-
-
-
-
-
-
-
1
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641995
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
267-278
1986
-
-
-
-
-
-
-
8
-
-
2
-
-
3
-
1
1
-
-
2
1
-
28
1
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
8
-
-
2
-
-
-
1
1
-
2
1
-
28
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
641996
Billich
Nucleoside phosphotransferase ...
Hordeum vulgare
Biol. Chem. Hoppe-Seyler
367
291-300
1986
6
-
-
-
-
1
3
-
-
6
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
6
-
-
-
-
-
1
-
3
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
642000
Tesoriere
The purification and propertie ...
Gallus gallus
Biochim. Biophys. Acta
786
231-244
1984
-
-
-
-
-
-
1
-
-
3
-
-
-
2
-
-
1
-
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
1
-
3
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
641994
Prasher
Nucleoside phosphotransferase ...
Hordeum vulgare
J. Biol. Chem.
257
4931-4939
1982
-
-
-
-
-
1
6
7
-
3
2
-
-
2
-
1
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
7
-
3
2
-
-
-
1
1
-
2
1
2
1
1
-
-
-
1
2
1
-
-
-
-
-
-
-
-
641999
Tesoriere
Kinetic properties of a nucleo ...
Gallus gallus
Biochim. Biophys. Acta
660
348-358
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
642001
Guranowski
Nucleoside phosphotransferase ...
Lupinus luteus
Biochim. Biophys. Acta
569
13-22
1979
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
2
1
-
32
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
641991
Brunngraber
Nucleoside phosphotransferase ...
Daucus carota
Methods Enzymol.
51
387-394
1978
1
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641997
Yuyama
Identification of true thymidi ...
Tetrahymena pyriformis, Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
-
-
-
-
-
-
-
-
1
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-
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2
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-
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2
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-
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-
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1
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-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641993
Chao
Nucleoside phosphotransferase ...
Pantoea agglomerans
J. Biol. Chem.
251
2330-2333
1976
-
-
-
-
-
5
5
-
2
1
2
-
-
2
-
-
1
-
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
2
1
2
-
-
-
-
1
-
-
1
1
17
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641138
Kit
Characterization of nucleoside ...
Gallus gallus
Arch. Biochem. Biophys.
169
66-76
1975
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641992
Rodgers
Nucleoside phosphotransferase ...
Daucus carota
J. Biol. Chem.
247
5448-5455
1972
-
-
-
-
-
-
3
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
641990
Brunngraber
Purification and properties of ...
Daucus carota
J. Biol. Chem.
242
4834-4840
1967
-
-
-
-
-
2
8
-
-
3
1
-
-
2
-
-
1
-
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
3
1
-
-
-
-
1
-
1
1
2
16
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-