Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner

Sandrini, M.P.; Clausen, A.R.; On, S.L.; Aarestrup, F.M.; Munch-Petersen, B.; Piskur, J.; J. Antimicrob. Chemother. 60, 510-520 (2007)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.027

-

2'-deoxycytidine

-

Bacillus cereus

0.027

-

deoxycytidine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.0332

-

2'-deoxyadenosine

-

Bacillus cereus

0.0332

-

deoxyadenosine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.0335

-

2',2'-difluorodeoxycytidine

ratio kcat/Km is 5100 per s and M

Bacillus cereus

0.0335

-

gemcitabine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

ATP + 2',2'-difluorodeoxycytidine

enzyme activates 2',2'-difluorodesoxycytidine in vivo

674219

Bacillus cereus

ADP + 2',2'-difluorodeoxycytidine 5'-phosphate

-

-

-

?

ATP + 2'-deoxyadenosine

-

674219

Bacillus cereus

ADP + 2'-deoxyadenosine 5'-phosphate

-

-

-

?

ATP + 2'-deoxycytidine

-

674219

Bacillus cereus

ADP + 2'-deoxycytidine 5'-phosphate

-

-

-

?

ATP + deoxyadenosine

-

674219

Bacillus cereus

ADP + dAMP

-

-

-

?

ATP + deoxycytidine

-

674219

Bacillus cereus

ADP + dCMP

-

-

-

?

ATP + gemcitabine

-

674219

Bacillus cereus

ADP + ?

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.17

-

gemcitabine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.55

-

deoxycytidine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

1.22

-

deoxyadenosine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

Cofactor

Commentary

Organism

Structure

ATP

-

Bacillus cereus

Cofactor

Commentary

Organism

Structure

ATP

-

Bacillus cereus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.027

-

2'-deoxycytidine

-

Bacillus cereus

0.027

-

deoxycytidine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.0332

-

2'-deoxyadenosine

-

Bacillus cereus

0.0332

-

deoxyadenosine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.0335

-

2',2'-difluorodeoxycytidine

ratio kcat/Km is 5100 per s and M

Bacillus cereus

0.0335

-

gemcitabine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

ATP + 2',2'-difluorodeoxycytidine

enzyme activates 2',2'-difluorodesoxycytidine in vivo

674219

Bacillus cereus

ADP + 2',2'-difluorodeoxycytidine 5'-phosphate

-

-

-

?

ATP + 2'-deoxyadenosine

-

674219

Bacillus cereus

ADP + 2'-deoxyadenosine 5'-phosphate

-

-

-

?

ATP + 2'-deoxycytidine

-

674219

Bacillus cereus

ADP + 2'-deoxycytidine 5'-phosphate

-

-

-

?

ATP + deoxyadenosine

-

674219

Bacillus cereus

ADP + dAMP

-

-

-

?

ATP + deoxycytidine

-

674219

Bacillus cereus

ADP + dCMP

-

-

-

?

ATP + gemcitabine

-

674219

Bacillus cereus

ADP + ?

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.17

-

gemcitabine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

0.55

-

deoxycytidine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

1.22

-

deoxyadenosine

in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS

Bacillus cereus

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

739204

Clausen

Plants salvage deoxyribonucleo ...

Arabidopsis thaliana, Solanum tuberosum

Nucleosides Nucleotides Nucleic Acids

33

291-295

2014

-

-

-

-

-

-

-

-

2

-

-

2

-

6

-

-

-

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-

1

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2

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4

-

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-

-

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-

-

-

2

-

-

2

-

-

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-

-

1

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

694483

Joachims

Inhibition of deoxynucleoside ...

Homo sapiens

Nucleosides Nucleotides Nucleic Acids

27

816-820

2008

-

1

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

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1

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-

-

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-

-

-

-

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1

-

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-

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1

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1

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-

-

-

-

674219

Sandrini

Nucleoside analogues are activ ...

Bacillus cereus

J. Antimicrob. Chemother.

60

510-520

2007

-

-

1

-

-

-

-

6

-

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1

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11

-

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1

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6

-

1

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3

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1

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1

1

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6

-

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1

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1

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-

-

6

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

675428

Welin

Structure-function analysis of ...

Mycoplasma mycoides

J. Mol. Biol.

366

1615-1623

2007

-

-

-

1

-

-

2

-

-

-

2

-

-

5

-

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1

-

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46

1

1

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1

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-

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-

1

2

-

-

-

2

-

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-

-

2

-

-

-

-

1

-

-

-

-

46

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

675444

Sandrini

Dictyostelium discoideum salva ...

Dictyostelium discoideum

J. Mol. Biol.

369

653-664

2007

-

-

1

1

-

-

-

5

-

-

1

1

-

7

-

-

-

-

-

-

-

-

10

1

1

-

-

-

6

-

-

-

1

-

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-

1

1

1

-

-

-

-

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6

-

-

1

1

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-

-

-

-

-

-

10

1

-

-

-

6

-

-

-

-

-

-

-

-

-

-

690459

Sandrini

Deoxyribonucleoside kinases ac ...

Staphylococcus aureus

Antimicrob. Agents Chemother.

51

2726-2732

2007

-

-

1

-

-

-

-

3

-

-

1

-

-

7

-

-

-

-

-

-

-

-

3

1

-

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-

3

-

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-

-

1

-

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-

-

-

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3

-

-

1

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3

1

-

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-

3

-

-

-

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-

-

-

-

-

-

672159

Pol

Kinetic mechanism of deoxyaden ...

Mycoplasma mycoides

Biochemistry

45

513-522

2006

-

-

-

-

-

-

-

10

-

-

-

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-

3

-

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6

-

1

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1

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1

-

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10

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-

6

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

676722

Katahira

Profiles of purine biosynthesi ...

Solanum tuberosum

Planta

225

115-126

2006

-

-

-

-

-

-

-

-

-

-

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-

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3

-

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-

-

1

1

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1

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-

-

-

-

-

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1

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-

-

-

-

1

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-

-

-

-

-

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1

1

-

1

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-

-

-

-

-

-

-

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-

-

-

-

660855

Krawiec

Inorganic tripolyphosphate (PP ...

Homo sapiens

Biochem. Biophys. Res. Commun.

301

192-197

2003

-

-

-

-

-

-

1

4

-

1

-

2

-

1

-

-

-

-

-

-

2

-

4

-

1

1

-

-

-

1

-

-

2

2

-

-

-

-

-

2

-

-

-

-

1

2

4

-

1

-

2

-

-

-

-

-

-

2

-

4

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

662985

Krawiec

Ability of adenosine-2'(3')-de ...

Homo sapiens

Nucleosides Nucleotides Nucleic Acids

22

1525-1529

2003

-

-

-

-

-

-

-

-

-

1

-

2

-

1

-

-

-

-

-

-

2

-

14

-

1

-

-

-

-

-

-

-

7

-

-

-

-

-

-

7

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

2

-

14

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

640561

Andersen

Deoxynucleoside kinases encode ...

Bacillus subtilis

J. Biol. Chem.

276

5518-5524

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-

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1

-

-

-

-

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1

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3

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1

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3

-

1

1

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1

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1

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1

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

3

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

641988

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Novel deoxynucleoside-phosphor ...

Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae

Mol. Microbiol.

42

1065-1073

2001

-

-

1

-

-

-

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7

-

-

2

-

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12

-

-

1

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10

1

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7

-

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1

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7

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2

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1

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-

-

10

1

-

-

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7

-

-

-

-

-

-

-

-

-

-

641989

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-

Protein engineering of deoxynu ...

Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396

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79-81

2001

-

-

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3

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4

-

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-

-

-

-

-

-

-

-

641984

Jenuth

Modeling of purine nucleoside ...

Mus musculus

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-

-

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-

-

-

-

-

1

-

2

-

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1

1

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1

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1

1

-

2

-

-

-

-

-

-

-

-

-

-

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-

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641987

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Metabolism of adenosine and de ...

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-

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-

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2

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2

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Directed mutagenesis of deoxyg ...

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-

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4

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1

5

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6

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-

640567

Ma

Cloning and expression of the ...

Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396

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270

6595-6601

1995

1

-

1

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1

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2

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4

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1

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4

1

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1

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1

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1

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4

1

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-

-

640566

Ikeda

Heterodimeric deoxynucleoside ...

Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396

Biochemistry

33

5328-5334

1994

-

-

-

-

-

1

4

-

-

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2

-

-

2

-

-

1

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1

2

2

2

1

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1

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4

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1

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1

2

2

1

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-

-

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-

-

641937

Kierdaszuk

Selective inactivation of the ...

Homo sapiens

Biochemistry

29

4109-4114

1990

-

-

-

-

-

1

1

1

-

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-

1

-

1

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1

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2

3

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1

1

5

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1

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1

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1

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1

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1

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2

3

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1

1

5

-

-

-

-

-

-

-

-

-

-

-

641980

Ikeda

Amino-terminal nucleotide-bind ...

Lactobacillus acidophilus

Biochemistry

27

8648-8652

1988

-

-

-

-

-

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3

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1

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1

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1

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2

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1

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641099

Carson

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Homo sapiens

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5677-5681

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641950

Deibler

Deoxynucleoside kinases from L ...

Lactobacillus acidophilus

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6

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641977

Baxter

Purification and detection of ...

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979-981

1977

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641976

Streeter

The phosphorylation of ribavir ...

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1974

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1

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1

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641981

Krygier

Mammalian deoxynucleoside kina ...

Bos taurus

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2745-2751

1971

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1

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1

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1

1

7

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641974

Krygier

The regulatory properties of d ...

Bos taurus

Biochim. Biophys. Acta

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578-580

1968

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1

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