Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus cereus |
expression in Escherichia coli | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
2'-deoxycytidine | - |
Bacillus cereus | |
0.027 | - |
deoxycytidine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus | |
0.0332 | - |
2'-deoxyadenosine | - |
Bacillus cereus | |
0.0332 | - |
deoxyadenosine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus | |
0.0335 | - |
gemcitabine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus | |
0.0335 | - |
2',2'-difluorodeoxycytidine | ratio kcat/Km is 5100 per s and M | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26200 | - |
SDS-PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q0H0H5 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose FF column chromatography | Bacillus cereus |
recombinant protein | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2',2'-difluorodeoxycytidine | enzyme activates 2',2'-difluorodesoxycytidine in vivo | Bacillus cereus | ADP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Bacillus cereus | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Bacillus cereus | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + deoxyadenosine | - |
Bacillus cereus | ADP + dAMP | - |
? | |
ATP + deoxycytidine | - |
Bacillus cereus | ADP + dCMP | - |
? | |
ATP + gemcitabine | - |
Bacillus cereus | ADP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dAK | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
gemcitabine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus | |
0.55 | - |
deoxycytidine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus | |
1.22 | - |
deoxyadenosine | in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus cereus |