BRENDA - Enzyme Database show
show all sequences of 2.7.1.76

Mammalian deoxynucleoside kinases. II. Deoxyadenosine kinase: purification and properties

Krygier, V.; Momparler, R.L.; J. Biol. Chem. 246, 2745-2751 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+
Bos taurus
Mg2+
required for activity
Bos taurus
Mn2+
activation, can replace Mg2+
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
gel filtration
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.073
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
-20°C, 60% glycerol, at least 3 months, slight loss of activity
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cytidine
approx. 18% of activity with deoxyadenosine
641981
Bos taurus
ADP + CMP
-
641981
Bos taurus
?
ATP + deoxyadenosine
-
641981
Bos taurus
ADP + dAMP
-
641981
Bos taurus
?
ATP + deoxycytidine
approx. 12% of activity with deoxyadenosine
641981
Bos taurus
ADP + dCMP
-
641981
Bos taurus
?
ATP + deoxyguanosine
-
641981
Bos taurus
ADP + dGMP
-
641981
Bos taurus
?
dTTP + deoxyadenosine
75% of activity with ATP
641981
Bos taurus
dTDP + dAMP
-
641981
Bos taurus
?
GTP + deoxyadenosine
-
641981
Bos taurus
GDP + dAMP
-
641981
Bos taurus
?
UTP + deoxyadenosine
90% of activity with ATP
641981
Bos taurus
UDP + dAMP
-
641981
Bos taurus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.7
8.8
approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+
Bos taurus
Mg2+
required for activity
Bos taurus
Mn2+
activation, can replace Mg2+
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
thymus
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.073
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 60% glycerol, at least 3 months, slight loss of activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cytidine
approx. 18% of activity with deoxyadenosine
641981
Bos taurus
ADP + CMP
-
641981
Bos taurus
?
ATP + deoxyadenosine
-
641981
Bos taurus
ADP + dAMP
-
641981
Bos taurus
?
ATP + deoxycytidine
approx. 12% of activity with deoxyadenosine
641981
Bos taurus
ADP + dCMP
-
641981
Bos taurus
?
ATP + deoxyguanosine
-
641981
Bos taurus
ADP + dGMP
-
641981
Bos taurus
?
dTTP + deoxyadenosine
75% of activity with ATP
641981
Bos taurus
dTDP + dAMP
-
641981
Bos taurus
?
GTP + deoxyadenosine
-
641981
Bos taurus
GDP + dAMP
-
641981
Bos taurus
?
UTP + deoxyadenosine
90% of activity with ATP
641981
Bos taurus
UDP + dAMP
-
641981
Bos taurus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.7
8.8
approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8
Bos taurus
Other publictions for EC 2.7.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739204
Clausen
Plants salvage deoxyribonucleo ...
Arabidopsis thaliana, Solanum tuberosum
Nucleosides Nucleotides Nucleic Acids
33
291-295
2014
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2
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2
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6
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1
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2
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2
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2
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1
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-
2
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-
-
-
-
-
-
-
-
-
-
-
-
-
694483
Joachims
Inhibition of deoxynucleoside ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
27
816-820
2008
-
1
-
-
-
-
1
-
-
-
-
-
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1
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1
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1
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1
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1
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674219
Sandrini
Nucleoside analogues are activ ...
Bacillus cereus
J. Antimicrob. Chemother.
60
510-520
2007
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-
1
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6
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1
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11
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1
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6
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3
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1
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1
1
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6
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1
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1
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6
-
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3
-
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675428
Welin
Structure-function analysis of ...
Mycoplasma mycoides
J. Mol. Biol.
366
1615-1623
2007
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-
-
1
-
-
2
-
-
-
2
-
-
5
-
-
1
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-
-
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46
1
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1
-
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1
2
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2
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2
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1
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46
1
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-
675444
Sandrini
Dictyostelium discoideum salva ...
Dictyostelium discoideum
J. Mol. Biol.
369
653-664
2007
-
-
1
1
-
-
-
5
-
-
1
1
-
7
-
-
-
-
-
-
-
-
10
1
-
-
-
6
-
-
-
1
-
-
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-
1
1
1
-
-
-
-
-
6
-
-
1
1
-
-
-
-
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-
-
-
10
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
690459
Sandrini
Deoxyribonucleoside kinases ac ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
51
2726-2732
2007
-
-
1
-
-
-
-
3
-
-
1
-
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7
-
-
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-
-
-
-
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3
1
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-
3
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1
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3
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1
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3
1
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3
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-
672159
Pol
Kinetic mechanism of deoxyaden ...
Mycoplasma mycoides
Biochemistry
45
513-522
2006
-
-
-
-
-
-
-
10
-
-
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3
-
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6
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1
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1
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10
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6
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676722
Katahira
Profiles of purine biosynthesi ...
Solanum tuberosum
Planta
225
115-126
2006
-
-
-
-
-
-
-
-
-
-
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-
3
-
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-
-
1
1
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1
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1
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1
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1
1
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1
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-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
-
-
-
-
-
1
4
-
1
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2
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1
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2
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4
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1
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1
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2
2
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2
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1
2
4
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1
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2
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2
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4
-
1
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1
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-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
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-
-
-
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2
-
14
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7
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7
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1
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2
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2
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14
-
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640561
Andersen
Deoxynucleoside kinases encode ...
Bacillus subtilis
J. Biol. Chem.
276
5518-5524
2001
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1
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1
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3
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1
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1
1
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1
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1
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3
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1
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1
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641988
Wang
Novel deoxynucleoside-phosphor ...
Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae
Mol. Microbiol.
42
1065-1073
2001
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1
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7
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2
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12
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1
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10
1
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7
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1
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7
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2
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1
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10
1
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7
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641989
Park
-
Protein engineering of deoxynu ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Food Sci. Nutr.
6
79-81
2001
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3
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4
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3
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641984
Jenuth
Modeling of purine nucleoside ...
Mus musculus
Biochem. Cell Biol.
74
219-225
1996
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-
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1
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2
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1
1
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1
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641987
Szabados
Metabolism of adenosine and de ...
Homo sapiens
Int. J. Biochem. Cell Biol.
28
1405-1414
1996
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2
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1
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640565
Hong
Directed mutagenesis of deoxyg ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6602-6606
1995
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4
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1
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640567
Ma
Cloning and expression of the ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6595-6601
1995
1
-
1
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1
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4
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1
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4
1
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1
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1
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1
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4
1
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640566
Ikeda
Heterodimeric deoxynucleoside ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
Biochemistry
33
5328-5334
1994
-
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1
4
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2
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2
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1
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1
2
2
1
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1
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4
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1
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1
2
2
1
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-
-
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-
-
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641937
Kierdaszuk
Selective inactivation of the ...
Homo sapiens
Biochemistry
29
4109-4114
1990
-
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1
1
1
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1
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1
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1
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2
3
-
1
1
5
-
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1
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1
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1
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1
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1
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2
3
-
1
1
5
-
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641980
Ikeda
Amino-terminal nucleotide-bind ...
Lactobacillus acidophilus
Biochemistry
27
8648-8652
1988
-
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3
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1
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1
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1
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1
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2
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1
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10
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641950
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Streeter
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1974
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4
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2
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1
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641974
Krygier
The regulatory properties of d ...
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1968
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11
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