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Literature summary for 2.7.1.76 extracted from
- Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors (1994), Biochemistry, 33, 5328-5334.
General Stability
| General Stability | Organism |
|---|---|
| dATP stabilizes | Lactobacillus acidophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dATP | kinetic, mechanism, protects corresponding active site against trypsin inactivation | Lactobacillus acidophilus |  |
| dCTP | kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP | Lactobacillus acidophilus | |
| dGTP | kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP | Lactobacillus acidophilus | |
| Trypsin | substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites | Lactobacillus acidophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27200 | - |
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE | Lactobacillus acidophilus |
| 29000 | - |
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE | Lactobacillus acidophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus acidophilus | - |
strain R-26 | - |
| Lactobacillus acidophilus ATCC 700396 | - |
strain R-26 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Lactobacillus acidophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| dATP stabilizes during storage | Lactobacillus acidophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxyadenosine | enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase | Lactobacillus acidophilus | ADP + dAMP | - |
? | |
| ATP + deoxyadenosine | enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase | Lactobacillus acidophilus ATCC 700396 | ADP + dAMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE | Lactobacillus acidophilus |
| More | both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits | Lactobacillus acidophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCyd kinase/dAdo kinase I | - |
Lactobacillus acidophilus |
| dGuo kinase/dAdo kinase II | - |
Lactobacillus acidophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Lactobacillus acidophilus |
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