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Literature summary for 2.7.1.74 extracted from

  • McSorley, T.; Ort, S.; Hazra, S.; Lavie, A.; Konrad, M.
    Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues (2008), FEBS Lett., 582, 720-724.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
S74A 6-8fold lower activity than wild-type Homo sapiens
S74D 2-4fold lower kcat values Homo sapiens
S74E mutation mimicking phosphorylation, results in 11fold increase in kcat value for deoxycytidine. Mutation has no effect on cellular localization Homo sapiens
S74Q no significant change in kcat for any of the substrates tested Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 2'-deoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
0.0059
-
 1-beta-D-arabinofuranosylcytosine wild-type, pH 7.5, 25°C Homo sapiens
0.0062
-
 2',2'-difluorodeoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
0.0089
-
 2'-deoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
0.015
-
 2',2'-difluorodeoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
0.024
-
 1-beta-D-arabinofuranosylcytosine mutant S74E, pH 7.5, 25°C Homo sapiens
0.1169
-
 2'-deoxyadenosine wild-type, pH 7.5, 25°C Homo sapiens
0.2458
-
 2'-deoxyguanosine wild-type, pH 7.5, 25°C Homo sapiens
0.518
-
 2'-deoxyadenosine mutant S74E, pH 7.5, 25°C Homo sapiens
0.602
-
 2'-deoxyguanosine mutant S74E, pH 7.5, 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus main localization of both wild-type and mutant S74E mimicking phosphorylated enzyme Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation at residue S74 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinofuranosylcytosine
-
 Homo sapiens ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
ATP + 2',2'-difluorodeoxycytidine
-
 Homo sapiens ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxy-CMP
-
 ?
ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 2'-deoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
0.04
-
 2'-deoxycytidine mutant S74A, pH 7.5, 25°C Homo sapiens
0.04
-
 2'-deoxycytidine mutant S74Q, pH 7.5, 25°C Homo sapiens
0.16
-
 2'-deoxycytidine mutant S74D, pH 7.5, 25°C Homo sapiens
0.3
-
 1-beta-D-arabinofuranosylcytosine mutant S74Q, pH 7.5, 25°C Homo sapiens
0.32
-
 1-beta-D-arabinofuranosylcytosine wild-type, pH 7.5, 25°C Homo sapiens
0.33
-
 1-beta-D-arabinofuranosylcytosine mutant S74A, pH 7.5, 25°C Homo sapiens
0.36
-
 2'-deoxyguanosine mutant S74E, pH 7.5, 25°C Homo sapiens
0.44
-
 2',2'-difluorodeoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
0.45
-
 2'-deoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
0.45
-
 2',2'-difluorodeoxycytidine mutant S74Q, pH 7.5, 25°C Homo sapiens
0.5
-
 2',2'-difluorodeoxycytidine mutant S74A, pH 7.5, 25°C Homo sapiens
0.57
-
 2'-deoxyguanosine mutant S74D, pH 7.5, 25°C Homo sapiens
0.57
-
 1-beta-D-arabinofuranosylcytosine mutant S74D, pH 7.5, 25°C Homo sapiens
0.65
-
 2'-deoxyguanosine mutant S74Q, pH 7.5, 25°C Homo sapiens
0.68
-
 2'-deoxyguanosine mutant S74A, pH 7.5, 25°C Homo sapiens
0.69
-
 2'-deoxyadenosine mutant S74E, pH 7.5, 25°C Homo sapiens
0.73
-
 2'-deoxyguanosine wild-type, pH 7.5, 25°C Homo sapiens
0.77
-
 2'-deoxyadenosine wild-type, pH 7.5, 25°C Homo sapiens
0.9
-
 2',2'-difluorodeoxycytidine mutant S74D, pH 7.5, 25°C Homo sapiens
0.9
-
 2'-deoxyadenosine mutant S74Q, pH 7.5, 25°C Homo sapiens
1
-
 2'-deoxyadenosine mutant S74D, pH 7.5, 25°C Homo sapiens
1.06
-
 1-beta-D-arabinofuranosylcytosine mutant S74E, pH 7.5, 25°C Homo sapiens
1.12
-
 2'-deoxyadenosine mutant S74A, pH 7.5, 25°C Homo sapiens
1.21
-
 2',2'-difluorodeoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens