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Literature summary for 2.7.1.74 extracted from

  • Godsey, M.H.; Ort, S.; Sabini, E.; Konrad, M.; Lavie, A.
    Structural basis for the preference of UTP over ATP in human deoxycytidine kinase: Illuminating the role of main-chain reorganization (2006), Biochemistry, 45, 452-461.
    View publication on PubMed
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed of engineered protein as His-tag fusion protein in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, in complex with deoxycytidine (dC) and UDP, and in the presence of deoxycytidine but the absence of UDP or ADP Homo sapiens

Protein Variants

Protein Variants Comment Organism
DEL65-79 construction of an enzyme variant lacking a flexible insert (residues 65-79) but having similar catalytic properties as the wild type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 2'-deoxycytidine less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.001
-
 2'-deoxycytidine less than 0.001 mM, wild type enzyme with ATP as cosubstrate Homo sapiens
0.001
-
 2'-deoxycytidine less than 0.001 mM, wild type enzyme with UTP as cosubstrate Homo sapiens
0.0014
-
 2'-deoxycytidine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
0.0015
-
 UTP residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.0023
-
 UTP wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.0029
-
 ATP residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.003
-
 1-beta-D-arabinofuranosylcytosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.003
-
 1-beta-D-arabinofuranosylcytosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.0035
-
 ATP wild type enzyme with 2'-deoxycytidine as cosubstrate Homo sapiens
0.0047
-
 UTP residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.007
-
 1-beta-D-arabinofuranosylcytosine wild type enzyme with ATP as cosubstrate Homo sapiens
0.01
-
 UTP wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.013
-
 1-beta-D-arabinofuranosylcytosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
0.013
-
 2'-deoxyadenosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.021
-
 2'-deoxyguanosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.053
-
 ATP residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.053
-
 2'-deoxyadenosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.087
-
 2'-deoxyguanosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.093
-
 ATP wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.1
-
 2'-deoxyadenosine wild type enzyme with ATP as cosubstrate Homo sapiens
0.231
-
 2'-deoxyguanosine wild type enzyme with ATP as cosubstrate Homo sapiens
0.415
-
 2'-deoxyadenosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
0.474
-
 2'-deoxyguanosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
 2 * 30500, calculated from the deduced amino acid sequence, crystal structure analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NTP + 1-beta-D-arabinofuranosylcytosine Homo sapiens antineoplastic agent NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
NTP + 2',3'-dideoxycytidine Homo sapiens antiviral agent NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxy-3'-thiacytidine Homo sapiens antiviral agent NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
NTP + 2'-deoxyadenosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides NDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
NTP + 2'-deoxycytidine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides NDP + 2'-deoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxyguanosine Homo sapiens rate-limiting reaction in the salvage of deoxyribonucleosides NDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
NTP + 2-chloro-2'-deoxyadenosine Homo sapiens i.e. cladribine, antineoplastic agent NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NTP + 1-beta-D-arabinofuranosylcytosine antineoplastic agent Homo sapiens NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
NTP + 2',3'-dideoxycytidine antiviral agent Homo sapiens NDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxy-3'-thiacytidine antiviral agent Homo sapiens NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
 ?
NTP + 2'-deoxyadenosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens NDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
NTP + 2'-deoxycytidine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens NDP + 2'-deoxycytidine 5'-phosphate
-
 ?
NTP + 2'-deoxyguanosine rate-limiting reaction in the salvage of deoxyribonucleosides Homo sapiens NDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
NTP + 2-chloro-2'-deoxyadenosine i.e. cladribine, antineoplastic agent Homo sapiens NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30500, calculated from the deduced amino acid sequence, crystal structure analysis Homo sapiens

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 ATP wild type enzyme with 2'-deoxycytidine as cosubstrate Homo sapiens
0.031
-
 UTP wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.033
-
 2'-deoxycytidine less than 0.001 mM, wild type enzyme with ATP as cosubstrate Homo sapiens
0.039
-
 ATP residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.041
-
 UTP residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate Homo sapiens
0.044
-
 2'-deoxycytidine less than 0.001 mM, wild type enzyme with UTP as cosubstrate Homo sapiens
0.049
-
 2'-deoxycytidine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
0.075
-
 UTP residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.088
-
 2'-deoxycytidine less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.093
-
 UTP wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.15
-
 1-beta-D-arabinofuranosylcytosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.17
-
 1-beta-D-arabinofuranosylcytosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.3
-
 1-beta-D-arabinofuranosylcytosine wild type enzyme with ATP as cosubstrate Homo sapiens
0.31
-
 2'-deoxyadenosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.32
-
 2'-deoxyguanosine residues 65-79 deletion mutant with UTP as cosubstrate Homo sapiens
0.33
-
 2'-deoxyadenosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.33
-
 2'-deoxyguanosine wild type enzyme with UTP as cosubstrate Homo sapiens
0.36
-
 ATP residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.4
-
 ATP wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate Homo sapiens
0.41
-
 1-beta-D-arabinofuranosylcytosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
1.7
-
 2'-deoxyadenosine wild type enzyme with ATP as cosubstrate Homo sapiens
2.2
-
 2'-deoxyguanosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens
2.6
-
 2'-deoxyguanosine wild type enzyme with ATP as cosubstrate Homo sapiens
2.8
-
 2'-deoxyadenosine residues 65-79 deletion mutant with ATP as cosubstrate Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
UTP preferred phosphoryl group donor Homo sapiens