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Literature summary for 2.7.1.74 extracted from

  • Datta, N.S.; Shewach, D.S.; Hurley, M.C.; Mitchell, B.S.; Fox, I.H.
    Human T-lymphoblast deoxycytidine kinase: purification and properties (1989), Biochemistry, 28, 114-123.
    View publication on PubMed
Search for more literature for 2.7.1.74

General Stability

General Stability Organism
dithiothreitol, MgCl2, ATP, KCl and glycerol stabilize Homo sapiens
very unstable at low protein concentrations Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-chloroadenosine
-
 Homo sapiens
Ba2+
-
 Homo sapiens
Cu2+
-
 Homo sapiens
cytidine deoxycytidine as substrate Homo sapiens
cytosine arabinoside deoxycytidine as substrate Homo sapiens
deoxyadenosine deoxycytidine Homo sapiens
deoxyguanosine deoxycytidine as substrate Homo sapiens
Dideoxycytidine
-
 Homo sapiens
HgCl2
-
 Homo sapiens
additional information little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview Homo sapiens
Nucleosides
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008 0.0015 deoxycytidine 37°C, pH 7.0 Homo sapiens
0.02 0.04 cytosine arabinoside pH 7.0, 37°C Homo sapiens
0.33 0.43 deoxyguanosine 37°C, pH 7.0 Homo sapiens
0.45
-
 cytidine 37°C, pH 7.0 Homo sapiens
0.5
-
 deoxyadenosine 37°C, pH 7.0 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Homo sapiens
Co2+ activation Homo sapiens
Co2+ about 30% as efficient as Mg2+ Homo sapiens
Fe2+ activation Homo sapiens
Fe2+ about 60% as efficient as Mg2+ Homo sapiens
Mg2+
-
 Homo sapiens
Mn2+ requirement Homo sapiens
Mn2+ about 90% as efficient as Mg2+ Homo sapiens
additional information no activation by Ba2+, Cu2+ Homo sapiens
additional information no activation by monovalent cations Homo sapiens
Ni2+ not Homo sapiens
Zn2+ activation Homo sapiens
Zn2+ about 30% as efficient as Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
 2 * 30500, SDS-PAGE Homo sapiens
60000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell leukemic T-lymphoblasts Homo sapiens
-
leukemia cell cell line MOLT-4 Homo sapiens
-
MOLT-4 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 cytidine as substrate, 37°C, pH 7.0 Homo sapiens
7.2
-
 deoxyguanosine as substrate, 37°C, pH 7.0 Homo sapiens
8
-
 37°C, pH 7.0 Homo sapiens
13.5
-
 deoxyadenosine as substrate, 37°C, pH 7.0 Homo sapiens

Storage Stability

Storage Stability Organism
-85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 5'-dCMP
-
 ?
ATP + cytidine
-
 Homo sapiens ADP + ?
-
 ?
CTP + 2'-deoxycytidine poor Homo sapiens CDP + 2'-deoxy-CMP
-
 ?
dGTP + 2'-deoxycytidine best phosphate donor Homo sapiens dGDP + 2'-deoxy-CMP
-
 ?
GTP + 2'-deoxycytidine as good as ATP Homo sapiens GDP + 2'-deoxy-CMP
-
 ?
NTP + cytosine arabinoside
-
 Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + deoxyadenosine
-
 Homo sapiens NDP + dAMP
-
 ?
NTP + deoxycytidine enzyme shows little specificity towards phosphate donor Homo sapiens NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: nucleoside diphosphates and their deoxy derivatives Homo sapiens NDP + dCMP
-
 ?
NTP + deoxyguanosine
-
 Homo sapiens NDP + dGMP
-
 ?
UTP + 2'-deoxycytidine poor Homo sapiens UDP + 2'-deoxy-CMP
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30500, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 broad Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.8 10 about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9 Homo sapiens