General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Bos taurus |
dithiothreitol stabilizes | Bos taurus |
SH-reducing agents stabilize | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dCMP | dTTP reverses; product inhibition, strong | Bos taurus | |
dCTP | no reversion by beta-gamma-methylene diphosphonate analogue of dTTP | Bos taurus | |
dTDP | - |
Bos taurus | |
Nucleoside analogs | related to deoxycytidine, deoxyadenosine or deoxyguanosine | Bos taurus | |
UDP | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Bos taurus | |
Co2+ | activation | Bos taurus | |
Fe2+ | activation | Bos taurus | |
Mg2+ | - |
Bos taurus | |
Mn2+ | requirement | Bos taurus | |
Ni2+ | activation | Bos taurus | |
Sr2+ | activation | Bos taurus | |
Zn2+ | activation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration, equilibrium density and sucrose density gradient centrifugation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxycytidine | Bos taurus | - |
ADP + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
preparative PAGE | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0206 | - |
37°C, pH 8.0 | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, in 5 mM DTT and 50% glycerol, stable | Bos taurus |
dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | - |
Bos taurus | ADP + 5'-dCMP | - |
? | |
ATP + cytidine | - |
Bos taurus | ADP + ? | - |
? | |
ATP + deoxycytidine | - |
Bos taurus | ADP + ? | - |
? | |
NTP + deoxyadenosine | - |
Bos taurus | NDP + dAMP | - |
? | |
NTP + deoxycytidine | no substrate: beta-gamma-methylene diphosphonate analogue of dTTP | Bos taurus | NDP + dCMP | - |
? | |
NTP + deoxycytidine | best acceptor substrate at low substrate concentrations | Bos taurus | NDP + dCMP | - |
? | |
NTP + deoxyguanosine | - |
Bos taurus | NDP + dGMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 | Bos taurus |
60 | - |
ATP, dCTP or glycerol stabilizes against heat inactivation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | nearly flat response to pH-variations | Bos taurus |