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Literature summary for 2.7.1.74 extracted from

  • Ives, D.H.; Wang, S.M.
    Deoxycytidine kinase from calf thymus (1978), Methods Enzymol., 51, 337-345.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin stabilizes Bos taurus
dithiothreitol stabilizes Bos taurus
SH-reducing agents stabilize Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
dCMP dTTP reverses; product inhibition, strong Bos taurus
dCTP no reversion by beta-gamma-methylene diphosphonate analogue of dTTP Bos taurus
dTDP
-
Bos taurus
Nucleoside analogs related to deoxycytidine, deoxyadenosine or deoxyguanosine Bos taurus
UDP
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Bos taurus
Co2+ activation Bos taurus
Fe2+ activation Bos taurus
Mg2+
-
Bos taurus
Mn2+ requirement Bos taurus
Ni2+ activation Bos taurus
Sr2+ activation Bos taurus
Zn2+ activation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration, equilibrium density and sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxycytidine Bos taurus
-
ADP + ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
preparative PAGE Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0206
-
37°C, pH 8.0 Bos taurus

Storage Stability

Storage Stability Organism
-20°C, in 5 mM DTT and 50% glycerol, stable Bos taurus
dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
Bos taurus ADP + 5'-dCMP
-
?
ATP + cytidine
-
Bos taurus ADP + ?
-
?
ATP + deoxycytidine
-
Bos taurus ADP + ?
-
?
NTP + deoxyadenosine
-
Bos taurus NDP + dAMP
-
?
NTP + deoxycytidine no substrate: beta-gamma-methylene diphosphonate analogue of dTTP Bos taurus NDP + dCMP
-
?
NTP + deoxycytidine best acceptor substrate at low substrate concentrations Bos taurus NDP + dCMP
-
?
NTP + deoxyguanosine
-
Bos taurus NDP + dGMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 Bos taurus
60
-
ATP, dCTP or glycerol stabilizes against heat inactivation Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10 nearly flat response to pH-variations Bos taurus