Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Bos taurus | |
dithiothreitol | activation | Bos taurus |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | not | Bos taurus | |
CMP | not | Bos taurus | |
cytidine | cytosine arabinoside as substrate | Bos taurus | |
cytosine arabinoside | deoxyguanosine as substrate | Bos taurus | |
dAMP | not | Bos taurus | |
dCDP | - |
Bos taurus | |
dCMP | - |
Bos taurus | |
dCTP | reversible by dTTP; reversible by UTP, dUTP | Bos taurus | |
deoxyadenosine | cytosine arabinoside or deoxyguanosine as substrate | Bos taurus | |
deoxycytidine | cytosine arabinoside as substrate; deoxyadenosine as substrate; deoxyguanosine as substrate | Bos taurus | |
deoxyguanosine | cytosine arabinoside as substrate | Bos taurus | |
deoxythymidine | cytosolic isozyme I, not | Bos taurus | |
dTDP | - |
Bos taurus | |
dUMP | not | Bos taurus | |
GDP | not | Bos taurus | |
GSSG | thiol reagents protect or reverse | Bos taurus | |
HgCl2 | strong, thiol reagents protect | Bos taurus | |
additional information | little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine | Bos taurus | |
N-ethylmaleimide | thiol reagents protect or reverse | Bos taurus | |
p-chloromercuribenzoate | thiol reagents protect or reverse | Bos taurus | |
UDP | - |
Bos taurus | |
UMP | not | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Bos taurus | |
Ca2+ | about 55% as efficient as Mg2+ | Bos taurus | |
CdCl2 | activation, in the absence of dithiothreitol | Bos taurus | |
Co2+ | activation | Bos taurus | |
Co2+ | about 40% as efficient as Mg2+ | Bos taurus | |
Fe2+ | activation | Bos taurus | |
Fe2+ | about 45% as efficient as Mg2+ | Bos taurus | |
Mg2+ | - |
Bos taurus | |
Mn2+ | requirement | Bos taurus | |
Mn2+ | about 80% as efficient as Mg2+ | Bos taurus | |
additional information | no activation by Ba2+, Cu2+ | Bos taurus | |
Ni2+ | activation | Bos taurus | |
Ni2+ | about 20% as efficient as Mg2+ | Bos taurus | |
Sr2+ | activation | Bos taurus | |
Sr2+ | about 15% as efficient as Mg2+ | Bos taurus | |
Zn2+ | activation | Bos taurus | |
Zn2+ | about 40% as efficient as Mg2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration, equilibrium density and sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
37°C, pH 8.0 | Bos taurus |
Storage Stability | Organism |
---|---|
-30°C, several months in the presence of 2-mercaptoethanol | Bos taurus |
4°C, if purified in the absence of 2-mercaptoethanol it loses most of its activity within 2 days, DTT restores | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | in the presence of DTT less effective than dUTP, UTP, dTTP or GTP, without DTT only less effective than dUTP | Bos taurus | ADP + 5'-dCMP | - |
? | |
dTTP + 2'-deoxycytidine | - |
Bos taurus | dTDP + 2'-deoxy-CMP | - |
? | |
dUTP + 2'-deoxycytidine | best phosphate donor | Bos taurus | dUDP + 2'-deoxy-CMP | - |
? | |
GTP + 2'-deoxycytidine | - |
Bos taurus | GDP + 2'-deoxy-CMP | - |
? | |
NTP + cytosine arabinoside | - |
Bos taurus | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
NTP + deoxyadenosine | - |
Bos taurus | NDP + dAMP | - |
? | |
NTP + deoxycytidine | - |
Bos taurus | NDP + dCMP | - |
? | |
NTP + deoxyguanosine | - |
Bos taurus | NDP + dGMP | - |
? | |
UTP + 2'-deoxycytidine | - |
Bos taurus | UDP + 2'-deoxy-CMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
at least 24 h, in the presence of dithiothreitol | Bos taurus |
60 | - |
dCTP enhances heat stability at pH 6, dithiothreitol slightly, not deoxycytidine or other nucleosides | Bos taurus |
60 | - |
t1/2: 7.9 min at pH 8, t1/2: 2 min at pH 6, unstable at pH 10 | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | broad plateau, with very slight optima at pH 6 and 10 | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
t1/2: 2 min at 60°C | Bos taurus |
8 | - |
moderately stable, t1/2: 7.9 min at 60°C | Bos taurus |
10 | - |
unstable at 60°C | Bos taurus |