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Literature summary for 2.7.1.71 extracted from
- Biochemical, kinetic, and computational structural characterization of shikimate kinase from methicillin-resistant Staphylococcus aureus (2019), Mol. Biotechnol., 61, 274-285 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3)pLysS cells | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000153 | - |
shikimate | at pH 7.6 and 25°C | Staphylococcus aureus |  |
| 0.224 | - |
ATP | at pH 7.6 and 25°C | Staphylococcus aureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 25 mM used in assay conditions | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + shikimate | Staphylococcus aureus | - |
ADP + 3-phosphoshikimate | - |
? | |
| ATP + shikimate | Staphylococcus aureus MRSA252 | - |
ADP + 3-phosphoshikimate | - |
? | |
| ATP + shikimate | Staphylococcus aureus ATCC BAA1720 | - |
ADP + 3-phosphoshikimate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | Q6GGG1 | - |
- |
| Staphylococcus aureus ATCC BAA1720 | Q6GGG1 | - |
- |
| Staphylococcus aureus MRSA252 | Q6GGG1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + shikimate | - |
Staphylococcus aureus | ADP + 3-phosphoshikimate | - |
? | |
| ATP + shikimate | - |
Staphylococcus aureus MRSA252 | ADP + 3-phosphoshikimate | - |
? | |
| ATP + shikimate | - |
Staphylococcus aureus ATCC BAA1720 | ADP + 3-phosphoshikimate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000, SDS-PAGE | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AroK | - |
Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 26 | after 8 h of incubation, the enzyme maintains its maximal activity in a range from 4 to 20°C, while at 25 and 26°C, it drops to 70% and 60%, respectively. A further increase of two degrees leads to the loss of 70% of the maximal activity, and at 30°C the enzyme is practically inactive | Staphylococcus aureus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Staphylococcus aureus | calculated from amino acid sequence | - |
5.1 |
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