Application | Comment | Organism |
---|---|---|
synthesis | the aroK enzyme knockout strain of Escherichia coli is useful for shikimate production | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
generation of knockout and antisense strain from Escherichia coli parent strain DH5alpha | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | inactivation of the aroK gene in an shikimate-producing Escherichia coli strain DHPYA-T7. In this strain, the aroL, ptsHIcrr and ydiB genes are deleted, and the tktA, glk, aroE and aroB genes are overexpressed. Accumulation of shikimate increases 2.69fold after aroK gene deletion and 1.29fold after antisense RNA interference. The activity of shikimate kinase in the knockout strain DHPYAAS-T7 is 0.21fold of that in the antisense strain DHPYAS-T7, while the accumulation of shikimate is 1.5fold in the knockout strain DHPYAAS-T7 compared to that in the antisense strain DHPYAS-T7 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Escherichia coli | - |
ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | Escherichia coli DHPYA-T7 | - |
ADP + 3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene aroK | - |
Escherichia coli DHPYA-T7 | - |
gene aroK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Escherichia coli | ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | - |
Escherichia coli DHPYA-T7 | ADP + 3-phosphoshikimate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | aroK gene inactivation in DHPYA-T7 leads to high shikimate accumulation, especially when this inactivation is caused by chromosomal deletion | Escherichia coli |