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Literature summary for 2.7.1.71 extracted from

  • Chen, K.; Dou, J.; Tang, S.; Yang, Y.; Wang, H.; Fang, H.; Zhou, C.
    Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli (2012), Biores. Technol., 119, 141-147.
    View publication on PubMed

Application

Application Comment Organism
synthesis the aroK enzyme knockout strain of Escherichia coli is useful for shikimate production Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
generation of knockout and antisense strain from Escherichia coli parent strain DH5alpha Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information inactivation of the aroK gene in an shikimate-producing Escherichia coli strain DHPYA-T7. In this strain, the aroL, ptsHIcrr and ydiB genes are deleted, and the tktA, glk, aroE and aroB genes are overexpressed. Accumulation of shikimate increases 2.69fold after aroK gene deletion and 1.29fold after antisense RNA interference. The activity of shikimate kinase in the knockout strain DHPYAAS-T7 is 0.21fold of that in the antisense strain DHPYAS-T7, while the accumulation of shikimate is 1.5fold in the knockout strain DHPYAAS-T7 compared to that in the antisense strain DHPYAS-T7 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + shikimate Escherichia coli
-
ADP + 3-phosphoshikimate
-
?
ATP + shikimate Escherichia coli DHPYA-T7
-
ADP + 3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene aroK
-
Escherichia coli DHPYA-T7
-
gene aroK
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
Escherichia coli ADP + 3-phosphoshikimate
-
?
ATP + shikimate
-
Escherichia coli DHPYA-T7 ADP + 3-phosphoshikimate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli

General Information

General Information Comment Organism
malfunction aroK gene inactivation in DHPYA-T7 leads to high shikimate accumulation, especially when this inactivation is caused by chromosomal deletion Escherichia coli