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Literature summary for 2.7.1.7 extracted from

  • Sabater, B.; Sebastian, J.; Asensio, C.
    Identification and properties of an inducible mannokinase from Streptomyces violaceoruber (1972), Biochim. Biophys. Acta, 284, 406-413.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-mannose
-
Streptomyces violaceoruber
alpha-Methyl-D-mannosamine
-
Streptomyces violaceoruber
D-glucose
-
Streptomyces violaceoruber
D-mannoheptulose
-
Streptomyces violaceoruber
D-mannosamine
-
Streptomyces violaceoruber
D-mannose 6-phosphate up to 20 mM no effect Streptomyces violaceoruber
lyxose L-isomer Streptomyces violaceoruber
MgADP- competitive Streptomyces violaceoruber
N-acetyl-D-mannosamine
-
Streptomyces violaceoruber

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
D-mannose
-
Streptomyces violaceoruber
0.4
-
mannoheptulose pH 7.5, 25°C Streptomyces violaceoruber
15
-
2-deoxy-D-mannose pH 7.5, 25°C Streptomyces violaceoruber
20
-
D-mannosamine pH 7.5, 25°C Streptomyces violaceoruber

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates with 40% of the activity with Mg2+ Streptomyces violaceoruber
Mg2+ required Streptomyces violaceoruber
Mg2+ optimal Mg2+/ATP ratio is 2 Streptomyces violaceoruber
Mg2+ Km: 0.4 mM Streptomyces violaceoruber
Mn2+ activates with 25% of the activity with Mg2+ Streptomyces violaceoruber

Organism

Organism UniProt Comment Textmining
Streptomyces violaceoruber
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces violaceoruber

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Streptomyces violaceoruber
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
-
Streptomyces violaceoruber

Storage Stability

Storage Stability Organism
0-2°C, 1 month, 25% loss of activity Streptomyces violaceoruber
15°C, 8 months, 25% loss of activity Streptomyces violaceoruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-mannose 25% of the activity with D-mannose Streptomyces violaceoruber ADP + 2-deoxy-D-mannose 6-phosphate
-
?
ATP + D-fructose no activity Streptomyces violaceoruber ADP + D-fructose 6-phosphate
-
?
ATP + D-fructose reaction is catalyzed by a separate enzyme, EC 2.7.1.4 Streptomyces violaceoruber ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose 80% of the activity with D-mannose Streptomyces violaceoruber ADP + D-glucose 6-phosphate
-
?
ATP + D-mannosamine 15% of the activity with D-mannose Streptomyces violaceoruber ADP + D-mannosamine 6-phosphate
-
?
ATP + D-mannose
-
Streptomyces violaceoruber ADP + mannose 6-phosphate
-
?
ATP + mannoheptulose 10% of the activity with D-mannose Streptomyces violaceoruber ADP + mannoheptulose 6-phosphate
-
?
CTP + 2-deoxy-D-mannose
-
Streptomyces violaceoruber CDP + 2-deoxy-D-mannose 6-phosphate
-
?
CTP + D-glucose
-
Streptomyces violaceoruber CDP + D-glucose 6-phosphate
-
?
CTP + D-mannosamine
-
Streptomyces violaceoruber CDP + D-mannosamine 6-phosphate
-
?
CTP + D-mannose 10% of the activity with ATP Streptomyces violaceoruber CDP + D-mannose 6-phosphate
-
?
CTP + mannoheptulose
-
Streptomyces violaceoruber CDP + mannoheptulose 6-phosphate
-
?
GTP + 2-deoxy-D-mannose
-
Streptomyces violaceoruber GDP + 2-deoxy-D-mannose 6-phosphate
-
?
GTP + D-glucose
-
Streptomyces violaceoruber GDP + D-glucose 6-phosphate
-
?
GTP + D-mannosamine
-
Streptomyces violaceoruber GDP + D-mannosamine 6-phosphate
-
?
GTP + D-mannose 40% of the activity with ATP Streptomyces violaceoruber GDP + D-mannose 6-phosphate
-
?
GTP + mannoheptulose
-
Streptomyces violaceoruber GDP + mannoheptulose 6-phosphate
-
?
ITP + 2-deoxy-D-mannose
-
Streptomyces violaceoruber IDP + 2-deoxy-D-mannose 6-phosphate
-
?
ITP + D-glucose
-
Streptomyces violaceoruber IDP + D-glucose 6-phosphate
-
?
ITP + D-mannosamine
-
Streptomyces violaceoruber IDP + D-mannosamine 6-phosphate
-
?
ITP + D-mannose 90% of the activity with ATP Streptomyces violaceoruber IDP + D-mannose 6-phosphate
-
?
ITP + mannoheptulose
-
Streptomyces violaceoruber IDP + mannoheptulose 6-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
5 min, crude extract, complete loss of activity Streptomyces violaceoruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces violaceoruber

pH Range

pH Minimum pH Maximum Comment Organism
7 9 activity falls rapidly outside the range 7-9 Streptomyces violaceoruber

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
MgADP- pH 7.5, 25°C Streptomyces violaceoruber
0.4
-
D-mannoheptulose pH 7.5, 25°C Streptomyces violaceoruber
4
-
D-glucose pH 7.5, 25°C Streptomyces violaceoruber
15
-
D-Lyxose pH 7.5, 25°C Streptomyces violaceoruber
15
-
2-deoxy-D-mannose pH 7.5, 25°C Streptomyces violaceoruber
20
-
D-mannosamine pH 7.5, 25°C Streptomyces violaceoruber
30
-
alpha-Methyl-D-mannosamine pH 7.5, 25°C Streptomyces violaceoruber
75
-
N-acetyl-D-mannosamine pH 7.5, 25°C Streptomyces violaceoruber