BRENDA - Enzyme Database
show all sequences of 2.7.1.7

D-fructose (D-mannose) kinase

Anderson, R.L.; Sapico, V.L.; Methods Enzymol. 42C, 39-43 (1975)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
D-fructose
pH 6.9, 25°C
Leuconostoc mesenteroides
0.4
-
D-mannose
pH 6.9, 25°C
Leuconostoc mesenteroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
sucrose density gradient centrifugation
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-fructose
Leuconostoc mesenteroides
enzyme initiates the metabolism of D-fructose and D-mannose
ADP + D-fructose 6-phosphate
-
-
?
ATP + D-mannose
Leuconostoc mesenteroides
enzyme initiates the metabolism of D-fructose and D-mannose
ADP + D-mannose 6-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leuconostoc mesenteroides
-
a single enzyme catalyzes phosphorylation of D-fructose and D-mannose
-
Purification (Commentary)
Commentary
Organism
-
Leuconostoc mesenteroides
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
76
-
-
Leuconostoc mesenteroides
Storage Stability
Storage Stability
Organism
0°C, 0.2 M (NH4)2SO4, pH 7.0, stable for several months
Leuconostoc mesenteroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose
D-mannose and D-fructose phosphorylated equally well
641481
Leuconostoc mesenteroides
ADP + D-fructose 6-phosphate
-
641481
Leuconostoc mesenteroides
?
ATP + D-fructose
enzyme initiates the metabolism of D-fructose and D-mannose
641481
Leuconostoc mesenteroides
ADP + D-fructose 6-phosphate
-
-
-
?
ATP + D-glucose
no activity with D-glucose or L-glucose
641481
Leuconostoc mesenteroides
ADP + D-glucose 6-phosphate
-
-
-
-
ATP + D-mannose
enzyme initiates the metabolism of D-fructose and D-mannose
641481
Leuconostoc mesenteroides
ADP + D-mannose 6-phosphate
-
-
-
?
ATP + D-mannose
D-mannose and D-fructose phosphorylated equally well
641481
Leuconostoc mesenteroides
ADP + mannose 6-phosphate
D-mannose 6-phosphate
641481
Leuconostoc mesenteroides
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
reaction with D-mannose
Leuconostoc mesenteroides
7
8.5
reaction with D-fructose
Leuconostoc mesenteroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
D-fructose
pH 6.9, 25°C
Leuconostoc mesenteroides
0.4
-
D-mannose
pH 6.9, 25°C
Leuconostoc mesenteroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
sucrose density gradient centrifugation
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-fructose
Leuconostoc mesenteroides
enzyme initiates the metabolism of D-fructose and D-mannose
ADP + D-fructose 6-phosphate
-
-
?
ATP + D-mannose
Leuconostoc mesenteroides
enzyme initiates the metabolism of D-fructose and D-mannose
ADP + D-mannose 6-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Leuconostoc mesenteroides
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
76
-
-
Leuconostoc mesenteroides
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 0.2 M (NH4)2SO4, pH 7.0, stable for several months
Leuconostoc mesenteroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose
D-mannose and D-fructose phosphorylated equally well
641481
Leuconostoc mesenteroides
ADP + D-fructose 6-phosphate
-
641481
Leuconostoc mesenteroides
?
ATP + D-fructose
enzyme initiates the metabolism of D-fructose and D-mannose
641481
Leuconostoc mesenteroides
ADP + D-fructose 6-phosphate
-
-
-
?
ATP + D-glucose
no activity with D-glucose or L-glucose
641481
Leuconostoc mesenteroides
ADP + D-glucose 6-phosphate
-
-
-
-
ATP + D-mannose
enzyme initiates the metabolism of D-fructose and D-mannose
641481
Leuconostoc mesenteroides
ADP + D-mannose 6-phosphate
-
-
-
?
ATP + D-mannose
D-mannose and D-fructose phosphorylated equally well
641481
Leuconostoc mesenteroides
ADP + mannose 6-phosphate
D-mannose 6-phosphate
641481
Leuconostoc mesenteroides
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
reaction with D-mannose
Leuconostoc mesenteroides
7
8.5
reaction with D-fructose
Leuconostoc mesenteroides
Other publictions for EC 2.7.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
641754
Mukai
Characterization and molecular ...
Arthrobacter sp., Arthrobacter sp. KM
Appl. Environ. Microbiol.
69
3849-3857
2003
1
-
1
-
-
-
-
4
-
7
1
-
-
12
-
-
1
1
-
-
4
-
8
1
1
-
1
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
4
-
7
1
-
-
-
-
1
-
-
4
-
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
641891
Gardiol
Transport and catabolism of D- ...
Sinorhizobium meliloti, Sinorhizobium meliloti L5-30
J. Bacteriol.
151
1069-1072
1982
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641893
Coulombel
Identification and kinetic stu ...
Streptomyces sp.
Biochim. Biophys. Acta
706
117-122
1982
-
-
-
-
-
-
2
2
-
1
1
1
-
2
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
4
2
-
1
1
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641481
Anderson
-
D-fructose (D-mannose) kinase ...
Leuconostoc mesenteroides
Methods Enzymol.
42C
39-43
1975
-
-
-
-
-
-
-
2
-
-
1
2
-
1
-
-
1
-
-
-
1
1
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
2
-
-
-
1
-
-
1
1
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
641892
Sabater
Identification and properties ...
Streptomyces violaceoruber
Biochim. Biophys. Acta
284
406-413
1972
-
-
-
-
-
-
9
4
-
3
-
-
-
2
-
-
1
-
-
1
1
2
22
-
-
-
1
-
1
1
-
-
8
-
-
-
-
-
-
-
-
-
-
9
8
4
-
3
-
-
-
-
-
1
-
1
1
2
22
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
641894
Sebastian
Purification and properties of ...
Escherichia coli
Arch. Biochem. Biophys.
151
227-233
1972
-
-
-
-
-
-
9
12
-
5
-
-
-
2
-
-
1
-
-
-
1
2
13
-
-
-
4
-
1
-
2
-
4
-
-
-
-
-
-
-
-
-
-
9
4
12
-
5
-
-
-
-
-
1
-
-
1
2
13
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-