Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | dynamic tubular, PI4KII | Drosophila melanogaster | 5768 | - |
Golgi apparatus | PI4KII | Drosophila melanogaster | 5794 | - |
additional information | PI4KII localizes to the Golgi and to a dynamic tubular endosomal network | Drosophila melanogaster | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | Drosophila melanogaster | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Drosophila melanogaster | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PI4KII | - |
Drosophila melanogaster |
type II phosphatidylinositol 4-kinase | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | in PI4KII mutants, mucin-containing glue granules fail to reach normal size, with glue protein aberrantly accumulating in enlarged Rab7-positive late endosomes. PI4KIIDELTA mutants reveal small glue granules of grossly normal morphology, PI4KIIDELTA cells also exhibited large vacuolated structures not observed in wild-type, phenotype, overview. Retromer localization and dynamics are altered in PI4KIIDELTA mutants | Drosophila melanogaster |
metabolism | formation of PI4KII-containing endosomal tubules is independent of retromer or AP-3 function, but retromer dynamics at LEs depends on PI4KII | Drosophila melanogaster |
physiological function | phosphatidylinositol 4-phosphate is a key regulator of membrane trafficking. Specific requirement for PI4KII emerged in larval salivary glands. In the Golgi, PI4KII is required for sorting of glue granule cargo and the granule-associated SNARE Snap24. In endosomes, it is required for normal retromer dynamics and for formation of tubular endosomes that are likely to be involved in retrieving Snap24 and lysosomal enzyme receptor protein from late endosomes to the trans-Golgi network. PI4KII in flies thus reveals a role for PI4KII in regulating the fidelity of granule protein trafficking in secretory tissues, PI4KII catalytic activity is required for granule biogenesis and normal endosome size, overview | Drosophila melanogaster |