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Literature summary for 2.7.1.67 extracted from
- Purification and chemical modification of a phosphatidylinositol kinase from sheep brain (1991), Eur. J. Biochem., 201, 249-255.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulates | Ovis aries |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'(3')-O-(2,4,6-Trinitrophenyl)ATP | - |
Ovis aries | |
| 5,5'-dithiobis(2-nitrobenzoate) | 0.03 mM, 10 min, 95% inhibition | Ovis aries | |
| adenosine | - |
Ovis aries | |
| ADP | - |
Ovis aries | |
| ATPgammaS | - |
Ovis aries | |
| deoxycholate | - |
Ovis aries | |
| Mn2+ | above 1 mM | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
phosphatidylinositol | pH 7.4, 30°C | Ovis aries | |
| 0.067 | - |
ATP | pH 7.4, 30°C | Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to | Ovis aries | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | most effective divalent cation activator, maximal activation at 10 mM | Ovis aries | |
| Mn2+ | can partially replace Mg2+ at concentrations below 1 mM | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Ovis aries |
| 150000 | - |
gel filtration | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.149 | - |
- |
Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Ovis aries | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | rapid decrease of activity above pH 8.0 and below pH 5.5 | Ovis aries |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
2'(3')-O-(2,4,6-Trinitrophenyl)ATP | pH 7.4, 30°C | Ovis aries | |
| 0.035 | - |
adenosine | pH 7.4, 30°C | Ovis aries | |
| 0.05 | - |
ADP | pH 7.4, 30°C | Ovis aries | |
| 0.18 | - |
ATPgammaS | pH 7.4, 30°C | Ovis aries | |
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