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Literature summary for 2.7.1.67 extracted from
- Membrane-associated phosphatidylinositol kinase from Saccharomyces cerevisiae (1983), J. Bacteriol., 156, 421-423.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium cholate | stimulates 3.8fold at 14 mM | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | - |
Saccharomyces cerevisiae | |
| K+ | - |
Saccharomyces cerevisiae | |
| Li+ | - |
Saccharomyces cerevisiae | |
| Na+ | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.071 | - |
phosphatidylinositol | pH 8.5, 30°C | Saccharomyces cerevisiae | |
| 0.21 | - |
ATP | pH 8.5, 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, maximal activity at 10 mM | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
S288c | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0933 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, about 50% loss of activity after 2 weeks | Saccharomyces cerevisiae |
| 8°C, 30% loss of activity after 4 h | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | active from pH 6.0 to pH 9.0 with Tris maleate buffer | Saccharomyces cerevisiae |
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