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Literature summary for 2.7.1.67 extracted from
- Phosphatidylinositol 4-kinase from yeast (1992), Methods Enzymol., 209, 183-189.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-bromo-ATP | no effect of enzyme activity in absence of spermidine, rather potent inhibitor in presence of stimulating amounts of spermidine | Saccharomyces cerevisiae |  |
| Ca2+ | - |
Saccharomyces cerevisiae | |
| heparin | - |
Saccharomyces cerevisiae | |
| N6-dimethylamine-adenosine 5'-triphosphate | no effect of enzyme activity in absence of spermidine, rather potent inhibitor in presence of stimulating amounts of spermidine | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | 0.5 | ATP | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, on storage the enzyme degrades to MW 30000 Da and 35000 Da but retains full activity, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae ade5 MATa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | sequential bi-bi reaction mechanism. Phosphatidylinositol binds prior to ATP, and phosphatidylinositol 4-phosphate is the first product released in the reaction | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.75 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae ade5 MATa | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, on storage the enzyme degrades to MW 30000 Da and 35000 Da but retains full activity, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
labile above | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.77 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 2.77 | - |
1-phosphatidyl-1D-myo-inositol | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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