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Literature summary for 2.7.1.66 extracted from

  • Sandermann, H.; Strominger, J.L.
    C55-isoprenoid alcohol phosphokinase: an enzyme soluble in organic solvents (1974), Methods Enzymol., 32, 439-446.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid cofactor phospholipid cofactor required Staphylococcus aureus

General Stability

General Stability Organism
remarkably stable towards SDS, 8 M urea and SH-reagents Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride
-
Staphylococcus aureus
NaCl above 0.5 M Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057
-
ATP
-
Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Staphylococcus aureus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 0.5 M, stimulates about 2-fold Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
apoprotein, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus the product of the reaction, C55-isoprenoid alcohol monophosphate functions in the biosynthesis of bacterial peptidoglycan and lipopolysaccharides ?
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein phospholipid cofactor required Staphylococcus aureus

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + C55-isoprenoid alcohol
-
Staphylococcus aureus ADP + C55-isoprenoid alcohol monophosphate
-
?
ATP + ficaprenol
-
Staphylococcus aureus ADP + ficaprenyl phosphate
-
?
ATP + solanesol poor substrate Staphylococcus aureus ADP + solanesyl phosphate
-
?
additional information the product of the reaction, C55-isoprenoid alcohol monophosphate functions in the biosynthesis of bacterial peptidoglycan and lipopolysaccharides Staphylococcus aureus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 17000, SDS-PAGE Staphylococcus aureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
30 min, about 30% loss of activity Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Staphylococcus aureus