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Literature summary for 2.7.1.63 extracted from

  • Liao, H.; Myung, S.; Zhang, Y.H.
    One-step purification and immobilization of thermophilic polyphosphate glucokinase from Thermobifida fusca YX: glucose-6-phosphate generation without ATP (2012), Appl. Microbiol. Biotechnol., 93, 1109-1117.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
orf Tfu_1811 overexpressed as protein fused with a family 3 cellulose-binding module in Escherichia coli strain BL21 (DE3) Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the recombinant cellulose-binding module fusion enzyme, which shows about 7.7fold increased catalytic turnover compared to the free enzyme Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
0.52
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
0.77
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
0.8
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
0.85
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca
0.94
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required as activator, best at 4 mM Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(phosphate)n + D-glucose Thermobifida fusca
-
(phosphate)n-1 + D-glucose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
orf Tfu_1811
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme fused with a family 3 cellulose-binding module 3.2fold from Escherichia coli strain BL21(DE3) in a one-step purification and immobilization on regenerated amorphous cellulose Thermobifida fusca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64.6
-
purified recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)n + D-glucose
-
Thermobifida fusca (phosphate)n-1 + D-glucose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
polyphosphate glucokinase
-
Thermobifida fusca
PPGK
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Thermobifida fusca

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 activity range, profile, overview. 40% of maximal activity at 50°C, maximal activity at 55°C, at pH 7.5, recombinant enzyme fused with a family 3 cellulose-binding module Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.6
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
39.7
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
92.6
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
96.9
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
129
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca
136
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
5 11 activity range, profile, overview. 40% of maximal activity at pH 7.5, 50°C, 31% at pH 9.0, 50°C, recombinant enzyme fused with a family 3 cellulose-binding module Thermobifida fusca

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
64.4
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
88.5
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C Thermobifida fusca
116
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
126
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C Thermobifida fusca
145
-
D-glucose recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca
151
-
D-glucose recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C Thermobifida fusca