Cloned (Comment) | Organism |
---|---|
orf Tfu_1811 overexpressed as protein fused with a family 3 cellulose-binding module in Escherichia coli strain BL21 (DE3) | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the recombinant cellulose-binding module fusion enzyme, which shows about 7.7fold increased catalytic turnover compared to the free enzyme | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
0.52 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
0.77 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
0.8 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
0.85 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca | |
0.94 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required as activator, best at 4 mM | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | Thermobifida fusca | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
orf Tfu_1811 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme fused with a family 3 cellulose-binding module 3.2fold from Escherichia coli strain BL21(DE3) in a one-step purification and immobilization on regenerated amorphous cellulose | Thermobifida fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64.6 | - |
purified recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | - |
Thermobifida fusca | (phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate glucokinase | - |
Thermobifida fusca |
PPGK | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Thermobifida fusca |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range, profile, overview. 40% of maximal activity at 50°C, maximal activity at 55°C, at pH 7.5, recombinant enzyme fused with a family 3 cellulose-binding module | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.6 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
39.7 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
92.6 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
96.9 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
129 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca | |
136 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | activity range, profile, overview. 40% of maximal activity at pH 7.5, 50°C, 31% at pH 9.0, 50°C, recombinant enzyme fused with a family 3 cellulose-binding module | Thermobifida fusca |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.4 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
88.5 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 30°C | Thermobifida fusca | |
116 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
126 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 7.5, 50°C | Thermobifida fusca | |
145 | - |
D-glucose | recombinant immobilized cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca | |
151 | - |
D-glucose | recombinant free cellulose-binding module fusion enzyme, pH 9.0, 50°C | Thermobifida fusca |