Literature summary for 2.7.1.63 extracted from

  • Mukai, T.; Kawai, S.; Matsukawa, H.; Matuo, Y.; Murata, K.
    Characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/ATP-glucomannokinase, of Arthrobacter sp. strain KM (2003), Appl. Environ. Microbiol., 69, 3849-3857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
glucose pH 7.0, 30°C Arthrobacter sp.
0.02
-
Hexametaphosphate pH 7.0, 30°C Arthrobacter sp.
0.2
-
ATP pH 7.0, 30°C Arthrobacter sp.
0.15
-
mannose pH 7.0, 30°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, maximal activition of polyphosphate-dependent glucokinase and ATP-dependent glucokinase activity, 5 mM Arthrobacter sp.
Mn2+ divalent cation required, 82% of the activity with Mg2+ for the polyphosphate-dependent activity and 88% of the activity with Mg2+ for ATP-dependent activity, 5 mM Arthrobacter sp.
Co2+ divalent cation required, 86% of the activity with Mg2+ for the polyphosphate-dependent activity and 30% of the activity with Mg2+ for ATP-dependent activity, 5 mM Arthrobacter sp.
Cu2+ divalent cation required, 11% of the activity with Mg2+ for the polyphosphate-dependent activity and no activation of ATP-dependent activity, 5 mM Arthrobacter sp.
Fe2+ divalent cation required, no activation of the polyphosphate-dependent activity and 15% of the activity with Mg2+ for ATP-dependent acticity, 5 mM Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. KM
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
reaction with polyphosphate and glucose Arthrobacter sp.
110
-
reaction with ATP and glucose Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP Arthrobacter sp. ?
-
?
additional information the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP Arthrobacter sp. KM ?
-
?
ATP + D-glucose
-
Arthrobacter sp. ADP + D-glucose 6-phosphate
-
?
ATP + D-glucose
-
Arthrobacter sp. KM ADP + D-glucose 6-phosphate
-
?
ATP + D-mannose about 30% of the activity with glucose Arthrobacter sp. ADP + D-mannose 6-phosphate
-
?
ATP + D-mannose about 30% of the activity with glucose Arthrobacter sp. KM ADP + D-mannose 6-phosphate
-
?
(phosphate)n + D-glucose
-
Arthrobacter sp. (phosphate)n-1 + D-glucose 6-phosphate
-
ir
(phosphate)n + D-glucose
-
Arthrobacter sp. KM (phosphate)n-1 + D-glucose 6-phosphate
-
ir
(phosphate)n + D-mannose about 40% of the activity with glucose Arthrobacter sp. (phosphate)n-1 + D-mannose 6-phosphate
-
?
(phosphate)n + D-mannose about 40% of the activity with glucose Arthrobacter sp. KM (phosphate)n-1 + D-mannose 6-phosphate
-
?
hexametaphosphate + D-glucose
-
Arthrobacter sp. pentametaphosphate + D-glucose 6-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, 50% loss of activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
polyphosphate- and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 25% of maximal activity, pH 9.5: about 35% of maximal activity, polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity Arthrobacter sp.