BRENDA - Enzyme Database
show all sequences of 2.7.1.63

Kinetic mechanisms of polyphosphate glucokinase from Mycobacterium tuberculosis

Hsieh, P.C.; Kowalczyk, T.H.; Phillips, N.F.; Biochemistry 35, 9772-9781 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
inhibits reaction with ATP
Mycobacterium tuberculosis
AMP
inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
ATP
at high concentrations competitive substrate inhibition with respect to glucose, ATP-dependent reaction
Mycobacterium tuberculosis
D-fructose 6-phosphate
inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
D-glucose 6-phosphate
inhibits reaction with polyphosphate and glucose
Mycobacterium tuberculosis
D-xylose
inhibits reaction with ATP; inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
D-glucose
pH 7.5, reaction with ATP
Mycobacterium tuberculosis
0.22
-
D-glucose
pH 8.6, reaction with ATP
Mycobacterium tuberculosis
0.88
-
ATP
pH 7.5
Mycobacterium tuberculosis
1.4
-
ATP
pH 8.6
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Reaction
Reaction
Commentary
Organism
(phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate
in the polyphosphate-dependent reaction the enzyme follows an ordered bi bi sequential mechanism with polyphosphate binding to the enzyme first and glucose 6-phosphate dissociating last. The ATP-dependent glucokinase reaction is consistent with an ordered bi bi sequential mechanism, with ATP binding to the enzyme first and glucose 6-phosphate leaving last
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(phosphate)n + D-glucose
polyphosphate is utilized nonprocessively with a preference for longer chains
641751
Mycobacterium tuberculosis
(phosphate)n-1 + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
-
641751
Mycobacterium tuberculosis
ADP + D-glucose 6-phosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
108
-
ATP
pH 7.5
Mycobacterium tuberculosis
108
-
glucose
pH 7.5
Mycobacterium tuberculosis
116
-
ATP
pH 8.6
Mycobacterium tuberculosis
116
-
glucose
pH 8.6
Mycobacterium tuberculosis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.6
-
ATP
pH 7.5
Mycobacterium tuberculosis
7.4
-
ATP
pH 8.6
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
inhibits reaction with ATP
Mycobacterium tuberculosis
AMP
inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
ATP
at high concentrations competitive substrate inhibition with respect to glucose, ATP-dependent reaction
Mycobacterium tuberculosis
D-fructose 6-phosphate
inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
D-glucose 6-phosphate
inhibits reaction with polyphosphate and glucose
Mycobacterium tuberculosis
D-xylose
inhibits reaction with ATP; inhibits reaction with polyphosphate and glucose or ATP and glucose
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.6
-
ATP
pH 7.5
Mycobacterium tuberculosis
7.4
-
ATP
pH 8.6
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
D-glucose
pH 7.5, reaction with ATP
Mycobacterium tuberculosis
0.22
-
D-glucose
pH 8.6, reaction with ATP
Mycobacterium tuberculosis
0.88
-
ATP
pH 7.5
Mycobacterium tuberculosis
1.4
-
ATP
pH 8.6
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(phosphate)n + D-glucose
polyphosphate is utilized nonprocessively with a preference for longer chains
641751
Mycobacterium tuberculosis
(phosphate)n-1 + D-glucose 6-phosphate
-
-
-
?
ATP + D-glucose
-
641751
Mycobacterium tuberculosis
ADP + D-glucose 6-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
108
-
ATP
pH 7.5
Mycobacterium tuberculosis
108
-
glucose
pH 7.5
Mycobacterium tuberculosis
116
-
ATP
pH 8.6
Mycobacterium tuberculosis
116
-
glucose
pH 8.6
Mycobacterium tuberculosis
Other publictions for EC 2.7.1.63
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737557
Romero-Rodríguez
Biochemistry and regulatory fu ...
Microlunatus phosphovorus, Thermobifida fusca
Arch. Biochem. Biophys.
577-578
1-10
2015
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2
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1
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2
1
1
-
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1
-
-
-
-
-
-
-
-
-
739015
Klemke
All1371 is a polyphosphate-dep ...
Anabaena sp., Anabaena sp. PCC 7120, Nostoc sp.
Microbiology
160
2807-2819
2014
-
-
2
-
-
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-
5
-
1
5
6
-
7
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1
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9
3
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5
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2
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5
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1
5
6
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1
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9
3
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5
-
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1
1
-
-
-
737862
Koide
Characterization of polyphosph ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2), Streptomyces coelicolor ATCC BAA-471
Biosci. Biotechnol. Biochem.
77
2322-2324
2013
-
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1
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3
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2
3
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5
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1
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9
1
1
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4
1
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1
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3
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2
3
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1
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-
9
1
1
-
-
4
1
-
-
-
-
-
-
-
-
-
721374
Liao
One-step purification and immo ...
Thermobifida fusca
Appl. Microbiol. Biotechnol.
93
1109-1117
2012
-
-
1
-
1
-
-
6
-
1
-
1
-
3
-
-
1
-
-
-
1
-
1
-
1
1
-
6
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
6
-
1
-
1
-
-
-
1
-
-
1
-
1
-
1
1
-
6
1
1
-
-
-
-
-
-
6
6
721367
Lindner
Cg2091 encodes a polyphosphate ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
87
703-713
2010
1
-
1
-
-
-
3
14
1
-
2
1
-
2
-
-
1
-
-
-
-
-
21
2
-
-
3
14
1
1
-
-
2
-
-
1
-
1
-
-
-
-
-
3
2
14
1
-
2
1
-
-
-
1
-
-
-
-
21
2
-
-
3
14
1
1
-
-
-
3
3
-
14
14
673712
Pallerla
Formation of volutin granules ...
Corynebacterium glutamicum
FEMS Microbiol. Lett.
243
133-140
2005
-
-
-
-
-
-
-
-
1
1
1
-
-
3
-
-
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-
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1
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1
1
1
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1
-
-
-
-
-
-
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-
-
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-
-
-
-
659420
Mukai
Crystal structure of bacterial ...
Arthrobacter sp., Arthrobacter sp. KM
J. Biol. Chem.
279
50591-50600
2004
-
-
1
1
-
-
-
-
-
-
-
2
-
4
-
-
1
1
-
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-
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2
1
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1
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1
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2
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641753
Tanaka
Strictly polyphosphate-depende ...
Microlunatus phosphovorus, Microlunatus phosphovorus NM-1
J. Bacteriol.
185
5654-5656
2003
-
-
-
-
-
-
-
3
-
4
2
-
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5
-
-
1
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10
1
1
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3
1
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3
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4
2
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1
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10
1
1
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3
1
-
-
-
-
-
-
-
-
-
641754
Mukai
Characterization and molecular ...
Arthrobacter sp., Arthrobacter sp. KM
Appl. Environ. Microbiol.
69
3849-3857
2003
-
-
1
-
-
-
-
4
-
5
-
-
-
13
-
-
1
-
-
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2
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10
-
1
-
1
-
1
1
-
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1
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-
-
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-
-
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4
-
5
-
-
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-
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1
-
-
2
-
10
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
641755
Phillips
Polyphosphate glucokinase ...
Acidipropionibacterium acidipropionici, Corynebacterium xerosis, Mycobacterium tuberculosis, Mycolicibacterium phlei, Nocardia minima, Propionibacterium freudenreichii subsp. shermanii
Prog. Mol. Subcell. Biol.
23
101-125
1999
-
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1
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-
9
-
-
5
1
-
6
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-
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-
12
3
-
-
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9
-
-
-
-
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-
1
-
-
-
-
-
-
-
9
-
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5
1
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-
-
-
-
12
3
-
-
-
9
-
-
-
-
-
-
-
-
-
-
641750
Kowalczyk
Initial rate and equilibrium i ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
35
6777-6785
1996
-
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4
6
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2
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1
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6
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6
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4
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6
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1
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6
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6
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641751
Hsieh
Kinetic mechanisms of polyphos ...
Mycobacterium tuberculosis
Biochemistry
35
9772-9781
1996
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6
4
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2
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1
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2
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4
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6
2
4
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2
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4
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641752
Hsieh
Cloning, expression, and chara ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
271
4909-4915
1996
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1
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2
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5
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1
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1
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1
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2
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1
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1
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2
1
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641746
Clark
-
Purification of polyphosphate ...
Propionibacterium freudenreichii subsp. shermanii
NATO ASI Ser. Ser. E
186
213-221
1990
-
-
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-
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1
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2
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1
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1
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1
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1
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1
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1
1
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-
641744
Girbal
-
Production, purification, prop ...
Mycobacterium gordonae, Mycolicibacterium aurum, Mycolicibacterium phlei, Mycolicibacterium smegmatis, Mycolicibacterium thermoresistibile
Enzyme Microb. Technol.
11
518-527
1989
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2
3
4
1
4
2
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5
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1
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1
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1
1
1
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1
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1
4
2
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1
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1
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5
1
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1
1
1
1
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641745
Pepin
Polyphosphate glucokinase from ...
Propionibacterium freudenreichii subsp. shermanii
J. Biol. Chem.
261
4476-4480
1986
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9
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2
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1
1
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1
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9
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1
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1
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641747
Szymona
Polyphosphate- and ATP-glucose ...
Nocardia minima
Acta Microbiol. Pol.
28
153-160
1979
-
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1
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1
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641748
Szymona
Multiple forms of polyphosphat ...
Mycobacterium jucho, Mycobacterium pellegrino, Mycobacterium sp.
Acta Microbiol. Pol.
27
73-76
1978
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4
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3
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641749
Szymona
A kinetic study on inorganic p ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Ra / ATCC 25177
Physiol. Chem. Phys.
6
393-404
1974
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2
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1
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10
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1
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2
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1
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1
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2
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641743
Szymona
Inorganic polyphosphate glucok ...
Mycolicibacterium phlei
Biochim. Biophys. Acta
85
283-295
1964
-
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2
1
2
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1
1
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2
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1
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1
1
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1
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1
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2
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1
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2
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1
1
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1
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1
1
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1
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1
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