General Stability | Organism |
---|---|
dialysis against distilled water for 10 h leads to decreased activity | Mycolicibacterium phlei |
KCl, 0.3 M, stabilizes | Mycolicibacterium phlei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tripolyphosphate | when present in excess together with Grahams salt at pH 7.2 | Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.175 | - |
(Phosphate)n | pH 8.5, 37°C, in terms of acid-labile phosphate | Mycolicibacterium phlei | |
0.28 | - |
D-glucose | pH 8.5, 37°C | Mycolicibacterium phlei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Mycolicibacterium phlei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113000 | - |
sucrose density gradient centrifugation | Mycolicibacterium phlei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Mycolicibacterium phlei |
Storage Stability | Organism |
---|---|
frozen, partially purified preparation, several weeks | Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | no utilization of triphosphate or tetrapolyphosphate | Mycolicibacterium phlei | (phosphate)n-1 + D-glucose 6-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mycolicibacterium phlei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
t1/2: 24 h at 3°C or at 37°C plus 0.3 M KCl | Mycolicibacterium phlei |
5 | 7 | 24 h stable at 3°C | Mycolicibacterium phlei |