BRENDA - Enzyme Database
show all sequences of 2.7.1.61

Acyl phosphate:hexose phosphotransferase (hexose phosphate:hexose phosphotransferase)

Anderson, R.L.; Kamel, M.Y.; Methods Enzymol. 9, 392-396 (1966)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
stable to repeated freezing and thawing
Klebsiella aerogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-mannose
inhibits phosphorylation of D-glucose, competitive to acetyl phosphate or D-mannose 6-phosphate
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
D-glucose
pH 7.5, 25°C
Klebsiella aerogenes
0.4
-
acetyl phosphate
carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C
Klebsiella aerogenes
2
-
D-ribose 5-phosphate
pH 7.5, 25°C
Klebsiella aerogenes
12
-
D-mannose
pH 7.5, 25°C
Klebsiella aerogenes
67
-
mannitol
pH 7.5, 25°C
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + acetyl phosphate
Klebsiella aerogenes
-
D-glucose 6-phosphate + acetate
-
-
-
D-glucose + acetyl phosphate
Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + acetate
-
-
-
D-glucose + D-mannose 6-phosphate
Klebsiella aerogenes
-
D-glucose 6-phosphate + D-mannose
-
-
-
D-glucose + D-mannose 6-phosphate
Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + D-mannose
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
PRL-R3
-
Klebsiella aerogenes PRL-R3
-
PRL-R3
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3
-
pH 7.5, 25°C
Klebsiella aerogenes
Storage Stability
Storage Stability
Organism
-20°C, stable
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-fructose 1-phosphate + D-fructose 6-phosphate + acetate
D-fructose 1-phosphate is the predominant product
636972
Klebsiella aerogenes
?
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + acetate
-
-
-
-
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + acetate
-
636972
Klebsiella aerogenes
?
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + acetate
-
-
-
-
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + acetate
-
636972
Klebsiella aerogenes PRL-R3
?
D-glucose + carbamoyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + carbamate
-
-
-
?
D-glucose + carbamoyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + carbamate
-
-
-
?
D-glucose + D-fructose 1-phosphate
15% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-fructose
-
-
-
?
D-glucose + D-fructose 6-phosphate
25% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-fructose
-
-
-
?
D-glucose + D-glucose 1-phosphate
5% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-glucose
-
-
-
?
D-glucose + D-mannose 6-phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-mannose
-
-
-
-
D-glucose + D-mannose 6-phosphate
71% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-mannose
-
-
-
r
D-glucose + D-mannose 6-phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + D-mannose
-
-
-
-
D-glucose + D-mannose 6-phosphate
71% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + D-mannose
-
-
-
r
D-glucose + D-ribose 5-phosphate
18% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-ribose
-
-
-
?
D-glucose + D-sorbitol 6-phosphate
2.5% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-sorbitol
-
-
-
?
D-mannitol + acetyl phosphate
-
636972
Klebsiella aerogenes
?
-
-
-
?
D-mannose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-mannose 6-phosphate + acetate
-
636972
Klebsiella aerogenes
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Klebsiella aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
pH 7.5: 50% of maximum activity, pH 9: maximum activity
Klebsiella aerogenes
General Stability (protein specific)
General Stability
Organism
stable to repeated freezing and thawing
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-mannose
inhibits phosphorylation of D-glucose, competitive to acetyl phosphate or D-mannose 6-phosphate
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
D-glucose
pH 7.5, 25°C
Klebsiella aerogenes
0.4
-
acetyl phosphate
carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C
Klebsiella aerogenes
2
-
D-ribose 5-phosphate
pH 7.5, 25°C
Klebsiella aerogenes
12
-
D-mannose
pH 7.5, 25°C
Klebsiella aerogenes
67
-
mannitol
pH 7.5, 25°C
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucose + acetyl phosphate
Klebsiella aerogenes
-
D-glucose 6-phosphate + acetate
-
-
-
D-glucose + acetyl phosphate
Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + acetate
-
-
-
D-glucose + D-mannose 6-phosphate
Klebsiella aerogenes
-
D-glucose 6-phosphate + D-mannose
-
-
-
D-glucose + D-mannose 6-phosphate
Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + D-mannose
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3
-
pH 7.5, 25°C
Klebsiella aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-fructose 1-phosphate + D-fructose 6-phosphate + acetate
D-fructose 1-phosphate is the predominant product
636972
Klebsiella aerogenes
?
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + acetate
-
-
-
-
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + acetate
-
636972
Klebsiella aerogenes
?
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + acetate
-
-
-
-
D-glucose + acetyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + acetate
-
636972
Klebsiella aerogenes PRL-R3
?
D-glucose + carbamoyl phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + carbamate
-
-
-
?
D-glucose + carbamoyl phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + carbamate
-
-
-
?
D-glucose + D-fructose 1-phosphate
15% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-fructose
-
-
-
?
D-glucose + D-fructose 6-phosphate
25% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-fructose
-
-
-
?
D-glucose + D-glucose 1-phosphate
5% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-glucose
-
-
-
?
D-glucose + D-mannose 6-phosphate
-
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-mannose
-
-
-
-
D-glucose + D-mannose 6-phosphate
71% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-mannose
-
-
-
r
D-glucose + D-mannose 6-phosphate
-
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + D-mannose
-
-
-
-
D-glucose + D-mannose 6-phosphate
71% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes PRL-R3
D-glucose 6-phosphate + D-mannose
-
-
-
r
D-glucose + D-ribose 5-phosphate
18% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-ribose
-
-
-
?
D-glucose + D-sorbitol 6-phosphate
2.5% of the activity with acetyl phosphate or carbamoyl phosphate
636972
Klebsiella aerogenes
D-glucose 6-phosphate + D-sorbitol
-
-
-
?
D-mannitol + acetyl phosphate
-
636972
Klebsiella aerogenes
?
-
-
-
?
D-mannose + acetyl phosphate
-
636972
Klebsiella aerogenes
D-mannose 6-phosphate + acetate
-
636972
Klebsiella aerogenes
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Klebsiella aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
pH 7.5: 50% of maximum activity, pH 9: maximum activity
Klebsiella aerogenes
Other publictions for EC 2.7.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636974
Casazza
Purification and initial rate ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
Biochemistry
16
3091-3097
1977
-
-
-
-
-
-
2
-
-
-
1
2
-
8
-
-
1
1
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
636973
Kamel
Acyl phosphate: hexose phospho ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
Arch. Biochem. Biophys.
120
322-331
1967
-
-
-
-
-
1
1
5
-
1
-
5
-
7
-
-
1
-
-
-
1
-
19
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
5
-
1
-
5
-
-
-
1
-
-
1
-
19
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
636972
Anderson
-
Acyl phosphate:hexose phosphot ...
Klebsiella aerogenes, Klebsiella aerogenes PRL-R3
Methods Enzymol.
9
392-396
1966
-
-
-
-
-
1
1
5
-
-
-
4
-
7
-
-
1
-
-
-
1
1
18
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
5
-
-
-
4
-
-
-
1
-
-
1
1
18
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-