General Stability | Organism |
---|---|
stable to repeated freezing and thawing | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-mannose | inhibits phosphorylation of D-glucose, competitive to acetyl phosphate or D-mannose 6-phosphate | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
D-glucose | pH 7.5, 25°C | Klebsiella aerogenes | |
0.4 | - |
acetyl phosphate | carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C | Klebsiella aerogenes | |
2 | - |
D-ribose 5-phosphate | pH 7.5, 25°C | Klebsiella aerogenes | |
12 | - |
D-mannose | pH 7.5, 25°C | Klebsiella aerogenes | |
67 | - |
mannitol | pH 7.5, 25°C | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + acetyl phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + acetate | - |
? | |
D-glucose + acetyl phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + acetate | - |
? | |
D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + D-mannose | - |
? | |
D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
PRL-R3 | - |
Klebsiella aerogenes PRL-R3 | - |
PRL-R3 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
pH 7.5, 25°C | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, stable | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + acetyl phosphate | - |
Klebsiella aerogenes | D-fructose 1-phosphate + D-fructose 6-phosphate + acetate | D-fructose 1-phosphate is the predominant product | ? | |
D-glucose + acetyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + acetate | - |
? | |
D-glucose + acetyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + acetate | - |
? | |
D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + carbamate | - |
? | |
D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + carbamate | - |
? | |
D-glucose + D-fructose 1-phosphate | 15% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
D-glucose + D-fructose 6-phosphate | 25% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
D-glucose + D-glucose 1-phosphate | 5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-glucose | - |
? | |
D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
? | |
D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
r | |
D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
? | |
D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
r | |
D-glucose + D-ribose 5-phosphate | 18% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-ribose | - |
? | |
D-glucose + D-sorbitol 6-phosphate | 2.5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-sorbitol | - |
? | |
D-mannitol + acetyl phosphate | - |
Klebsiella aerogenes | ? | - |
? | |
D-mannose + acetyl phosphate | - |
Klebsiella aerogenes | D-mannose 6-phosphate + acetate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: 50% of maximum activity, pH 9: maximum activity | Klebsiella aerogenes |