Literature summary for 2.7.1.60 extracted from

Aretz, J.; Wratil, P.; Wamhoff, E.; Nguyen, H.; Reutter, W.; Rademacher, C.
Fragment screening of N-acetylmannosamine kinase reveals noncarbohydrate inhibitors (2016), Can. J. Chem., 94, 920-926 .

No PubMed abstract available

Search for more literature for 2.7.1.60

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,3-dimethyl-4-(trifluoromethyl)-1,7-dihydro-6H-pyrazolo[3,4-b]pyridin-6-one	-	Homo sapiens
1,6-dimethyl-2-oxo-4-(trifluoromethyl)-1,2-dihydropyridine-3-carbonitrile	-	Homo sapiens
2-ethyl-4-(trifluoromethyl)-2,7-dihydro-6H-pyrazolo[3,4-b]pyridin-6-one	-	Homo sapiens
2-hydroxy-6-propyl-4-(trifluoromethyl)pyridine-3-carbonitrile	-	Homo sapiens
2-methoxy-4-(trifluoromethyl)pyridine-3-carboxylic acid	-	Homo sapiens
3-acetyl-5-chloropyridine-2-carboxylic acid	-	Homo sapiens
4-(trifluoromethyl)picolinic acid	-	Homo sapiens
4-(trifluoromethyl)pyridine-2-carboxylic acid	also named 4-(trifluoromethyl)picolinic acid	Homo sapiens
4-oxo-1,4-dihydropyridine-2,6-dicarboxylic acid	-	Homo sapiens
5-(cyanomethyl)pyridine-2-carboxylic acid	-	Homo sapiens
5-carbamoyl-3-chloropyridine-2-carboxylic acid	-	Homo sapiens
6-(cyclopentylcarbamoyl)pyridine-2-carboxylic acid	-	Homo sapiens
6-(pyrrolidin-1-ylcarbonyl)pyridine-2-carboxylic acid	-	Homo sapiens
6-acetylpicolinic acid	-	Homo sapiens
6-acetylpyridine-2-carboxylic acid	also named 6-acetylpicolinic acid	Homo sapiens
6-carbamoylpyridine-2-carboxylic acid	-	Homo sapiens
6-cyclopropyl-4-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-3-amine	-	Homo sapiens
6-methyl-4-(trifluoromethyl)pyridin-2(1H)-one	-	Homo sapiens
methyl 5,6-diaminopyridine-2-carboxylate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	65 mM used in assay conditions	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	Homo sapiens	-	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	-	Homo sapiens	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
MNK	-	Homo sapiens
N-acetylmannosamine kinase	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.076	-	at pH 8.0 and 37°C	Homo sapiens	6-carbamoylpyridine-2-carboxylic acid
0.078	-	at pH 8.0 and 37°C	Homo sapiens	4-oxo-1,4-dihydropyridine-2,6-dicarboxylic acid
0.08	-	at pH 8.0 and 37°C	Homo sapiens	6-(pyrrolidin-1-ylcarbonyl)pyridine-2-carboxylic acid
0.4	-	at pH 8.0 and 37°C	Homo sapiens	1,6-dimethyl-2-oxo-4-(trifluoromethyl)-1,2-dihydropyridine-3-carbonitrile
0.7	-	at pH 8.0 and 37°C	Homo sapiens	3-acetyl-5-chloropyridine-2-carboxylic acid
0.7	-	at pH 8.0 and 37°C	Homo sapiens	6-acetylpyridine-2-carboxylic acid
0.7	-	at pH 8.0 and 37°C	Homo sapiens	6-acetylpicolinic acid
0.8	-	at pH 8.0 and 37°C	Homo sapiens	5-carbamoyl-3-chloropyridine-2-carboxylic acid
0.8	-	at pH 8.0 and 37°C	Homo sapiens	methyl 5,6-diaminopyridine-2-carboxylate
0.9	-	at pH 8.0 and 37°C	Homo sapiens	2-methoxy-4-(trifluoromethyl)pyridine-3-carboxylic acid
0.9	-	at pH 8.0 and 37°C	Homo sapiens	4-(trifluoromethyl)pyridine-2-carboxylic acid
0.9	-	at pH 8.0 and 37°C	Homo sapiens	4-(trifluoromethyl)picolinic acid
1.6	-	at pH 8.0 and 37°C	Homo sapiens	6-methyl-4-(trifluoromethyl)pyridin-2(1H)-one
2.5	-	IC50 above 2.5 mM, at pH 8.0 and 37°C	Homo sapiens	1,3-dimethyl-4-(trifluoromethyl)-1,7-dihydro-6H-pyrazolo[3,4-b]pyridin-6-one
2.5	-	IC50 above 2.5 mM, at pH 8.0 and 37°C	Homo sapiens	2-ethyl-4-(trifluoromethyl)-2,7-dihydro-6H-pyrazolo[3,4-b]pyridin-6-one
2.5	-	IC50 above 2.5 mM, at pH 8.0 and 37°C	Homo sapiens	2-hydroxy-6-propyl-4-(trifluoromethyl)pyridine-3-carbonitrile
2.5	-	IC50 above 2.5 mM, at pH 8.0 and 37°C	Homo sapiens	5-(cyanomethyl)pyridine-2-carboxylic acid
2.5	-	IC50 above 2.5 mM, at pH 8.0 and 37°C	Homo sapiens	6-cyclopropyl-4-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-3-amine
3.7	-	at pH 8.0 and 37°C	Homo sapiens	6-(cyclopentylcarbamoyl)pyridine-2-carboxylic acid

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Release 2023.1 (January 2023)