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Literature summary for 2.7.1.60 extracted from

  • Penner, J.; Mantey, L.R.; Elgavish, S.; Ghaderi, D.; Cirak, S.; Berger, M.; Krause, S.; Lucka, L.; Voit, T.; Mitrani-Rosenbaum, S.; Hinderlich, S.
    Influence of UDP-GlcNAc 2-epimerase/ManNAc kinase mutant proteins on hereditary inclusion body myopathy (2006), Biochemistry, 45, 2968-2977.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A524V 30% ManNAc kinase activity of the wild type enzyme Homo sapiens
A630T 40% ManNAc kinase activity of the wild type enzyme Homo sapiens
A631T 75% ManNAc kinase activity of the wild type enzyme Homo sapiens
A631V 15% ManNAc kinase activity of the wild type enzyme Homo sapiens
A631V 65% ManNAc kinase activity of the wild type enzyme Homo sapiens
C13S 105% ManNAc kinase activity of the wild type enzyme Homo sapiens
C303V 60% ManNAc kinase activity of the wild type enzyme Homo sapiens
C303X no ManNAc kinase activity Homo sapiens
D176V 85% ManNAc kinase activity of the wild type enzyme Homo sapiens
D378Y 45% ManNAc kinase activity of the wild type enzyme Homo sapiens
F528C 35% ManNAc kinase activity of the wild type enzyme Homo sapiens
F537I 60% ManNAc kinase activity of the wild type enzyme Homo sapiens
G576E 15% ManNAc kinase activity of the wild type enzyme Homo sapiens
G708S 5% ManNAc kinase activity of the wild type enzyme Homo sapiens
H132Q 50% ManNAc kinase activity of the wild type enzyme Homo sapiens
I200F 75% ManNAc kinase activity of the wild type enzyme Homo sapiens
I472T 5% ManNAc kinase activity of the wild type enzyme Homo sapiens
I587T 35% ManNAc kinase activity of the wild type enzyme Homo sapiens
M712T 70% ManNAc kinase activity of the wild type enzyme Homo sapiens
N519S 20% ManNAc kinase activity of the wild type enzyme Homo sapiens
R11W 40% ManNAc kinase activity of the wild type enzyme Homo sapiens
R177C 80% ManNAc kinase activity of the wild type enzyme Homo sapiens
V331A 115% ManNAc kinase activity of the wild type enzyme Homo sapiens
V572L 10% ManNAc kinase activity of the wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and PD-10 gel column filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-mannosamine
-
Homo sapiens ADP + N-acetyl-D-mannosamine 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
UDP-GlcNAc 2-epimerase/ManNAc kinase
-
Homo sapiens