Cloned (Comment) | Organism |
---|---|
expressed with or without His-tag in Escherichia coli BL21 Star(DE3), Saccharomyces cerevisiae, Pichia pastoris, High five, Sf9, and Sf-900 cells, low yield in Escherichia coli and yeast, high expression of enzyme in High five, and Sf-900 cells | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
6 * 79000 | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acyl-D-mannosamine | Rattus norvegicus | key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes from all species, purification from Sf-900 can yield milligram amounts of soluble, active protein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.9 | - |
purified enzyme from Sf-900 cells, pH 8.1, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, enzyme can be stored without substantial loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acyl-D-mannosamine | key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids | Rattus norvegicus | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 79000 | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
UDP-GlcNAc 2-epimerase/ManNAc kinase | - |
Rattus norvegicus |
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | two different enzyme activities combined in a single bifunctional enzyme | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | pH above 12 results in almost complete disaggregation and complete loss of activity | Rattus norvegicus |
6 | - |
pH below 6 results in almost complete disaggregation and complete loss of activity | Rattus norvegicus |