Cloned (Comment) | Organism |
---|---|
overexpression of active enzyme by using the baculovirus/Sf9 system | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D413K | loss of kinase activity | Rattus norvegicus |
D413L | loss of kinase activity | Rattus norvegicus |
D413N | loss of kinase activity | Rattus norvegicus |
H110A | loss of epimerase activity | Rattus norvegicus |
H132A | loss of epimerase activity | Rattus norvegicus |
H155A | loss of epimerase activity | Rattus norvegicus |
H157A | loss of epimerase activity | Rattus norvegicus |
H45A | loss of epimerase activity | Rattus norvegicus |
R420M | loss of kinase activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration, SDS-PAGE | Rattus norvegicus |
450000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-mannosamine | Rattus norvegicus | involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.84 | - |
recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-mannosamine | involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis | Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, self associates as a dimer, gel filtration, SDS-PAGE, catalyzes only phosphorylation of N-acetylmannosamine, incubation with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer | Rattus norvegicus |
hexamer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
EC 5.1.3.14/EC 2.7.1.60 | - |
Rattus norvegicus |
UDP-GlcNAc-epimerase/ManNAc kinase | - |
Rattus norvegicus |