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Literature summary for 2.7.1.60 extracted from

  • Effertz, K.; Hinderlich, S.; Reutter, W.
    Selective loss of either the epimerase or kinase activity of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase due to site-directed mutagenesis based on sequence alignments (1999), J. Biol. Chem., 274, 28771-28778.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of active enzyme by using the baculovirus/Sf9 system Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D413K loss of kinase activity Rattus norvegicus
D413L loss of kinase activity Rattus norvegicus
D413N loss of kinase activity Rattus norvegicus
H110A loss of epimerase activity Rattus norvegicus
H132A loss of epimerase activity Rattus norvegicus
H155A loss of epimerase activity Rattus norvegicus
H157A loss of epimerase activity Rattus norvegicus
H45A loss of epimerase activity Rattus norvegicus
R420M loss of kinase activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration, SDS-PAGE Rattus norvegicus
450000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-mannosamine Rattus norvegicus involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis ADP + N-acetyl-D-mannosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-mannosamine involved in N-acetylneuraminic acid metabolism, key enzyme in N-acetylneuraminic acid biosynthesis Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
?
ATP + N-acetyl-D-mannosamine
-
Rattus norvegicus ADP + N-acetyl-D-mannosamine-6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 75000, self associates as a dimer, gel filtration, SDS-PAGE, catalyzes only phosphorylation of N-acetylmannosamine, incubation with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer Rattus norvegicus
hexamer
-
Rattus norvegicus

Synonyms

Synonyms Comment Organism
EC 5.1.3.14/EC 2.7.1.60
-
Rattus norvegicus
UDP-GlcNAc-epimerase/ManNAc kinase
-
Rattus norvegicus