General Stability | Organism |
---|---|
hexameric enzyme is completely stable with 0.1 mM UDP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
N-acetyl-D-mannosamine | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
0.121 | - |
N-acetyl-D-mannosamine | pH 7.5, 37°C, dimer | Rattus norvegicus | |
1.18 | - |
ATP | pH 7.5, 37°C, hexamer | Rattus norvegicus | |
1.67 | - |
ATP | pH 7.5, 37°C, dimer | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration, SDS-PAGE | Rattus norvegicus |
150000 | - |
gel filtration, ion of the monomeric 75000 Da protein self associate as dimer | Rattus norvegicus |
450000 | - |
gel filtration, ion of the monomeric 75000 Da protein self associate as hexamer | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-mannosamine | Rattus norvegicus | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | Rattus norvegicus Wistar | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar | - |
Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal mucosa | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
salivary gland | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.114 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, no loss of activity observed for several months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus Wistar | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | - |
Rattus norvegicus Wistar | ADP + N-acetyl-D-mannosamine-6-phosphate | - |
? | |
additional information | UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis | Rattus norvegicus | ? | - |
? | |
additional information | UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis | Rattus norvegicus Wistar | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, self associates as a dimer, gel filtration, SDS-PAGE, catalyzes only phosphorylation of N-acetylmannosamine, incubation with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer | Rattus norvegicus |
hexamer | 6 * 75000, self associates as a hexamer, gel filtration, SDS-PAGE, catalyzes both enzyme activities, UDP-GlcNAc-epimerase/ManNAc kinase | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ManAc kinase | - |
Rattus norvegicus |
UDP-GlcNAc-epimerase/ManNAc kinase | - |
Rattus norvegicus |