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Literature summary for 2.7.1.6 extracted from
- Characterisation of a bacterial galactokinase with high activity and broad substrate tolerance for chemoenzymatic synthesis of 6-aminogalactose-1-phosphate and analogues (2018), ChemBioChem, 19, 388-394 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | LgGalK and galactose oxidase variant M1 are combined in a one-pot, two-step system to synthesize 6-oxogalactose-1-phosphate and 6-oxo-2-fluorogalactose-1-phosphate, which are subsequently used to produce a panel of 30 substituted 6-aminogalactose-1-phosphate derivatives by chemical reductive amination in a one-pot, three-step chemoenzymatic process | Leminorella grimontii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Leminorella grimontii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Leminorella grimontii |  |
| EDTA | - |
Leminorella grimontii | |
| Zn2+ | - |
Leminorella grimontii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
alpha-D-galactose | pH 9.0, 50°C | Leminorella grimontii | |
| 1.5 | - |
ATP | pH 9.0, 50°C | Leminorella grimontii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Leminorella grimontii | |
| Fe2+ | activates | Leminorella grimontii | |
| Mg2+ | activates. Optimum Mg2+ concentration is around 1 mm, with a very modest decrease in activity as the Mg2+ concentration increases | Leminorella grimontii | |
| Ni2+ | activates | Leminorella grimontii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Leminorella grimontii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leminorella grimontii | A0A085HKM4 | - |
- |
| Leminorella grimontii DSM 5078 | A0A085HKM4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leminorella grimontii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-deoxy-3-fluorogalactose | 27% conversion | Leminorella grimontii | ADP + 3-deoxy-3-fluorogalactose 1-phosphate | - |
? | |
| ATP + 3-deoxy-3-fluorogalactose | 27% conversion | Leminorella grimontii DSM 5078 | ADP + 3-deoxy-3-fluorogalactose 1-phosphate | - |
? | |
| ATP + 3-deoxy-D-galactose | 19% conversion | Leminorella grimontii | ADP + 3-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + 4-deoxy-4-fluorogalactose | 21% conversion | Leminorella grimontii | ADP + 4-deoxy-4-fluorogalactose 1-phosphate | - |
? | |
| ATP + 6-deoxy-6-fluorogalactose | 28% conversion | Leminorella grimontii | ADP + 6-deoxy-6-fluorogalactose 1-phosphate | - |
? | |
| ATP + 6-deoxy-D-galactose | 57% conversion | Leminorella grimontii | ADP + 6-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + 6-deoxy-D-galactose | 57% conversion | Leminorella grimontii DSM 5078 | ADP + 6-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | 72% conversion | Leminorella grimontii | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | 72% conversion | Leminorella grimontii DSM 5078 | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | 68% conversion | Leminorella grimontii | ADP + alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactosamine | 68% conversion | Leminorella grimontii DSM 5078 | ADP + alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + D-talose | 41% conversion | Leminorella grimontii | ADP + alpha-D-talose 1-phosphate | - |
? | |
| ATP + D-talose | 41% conversion | Leminorella grimontii DSM 5078 | ADP + alpha-D-talose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Leminorella grimontii |
| ? | x * 42000, calculated from sequence | Leminorella grimontii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GALK | - |
Leminorella grimontii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Leminorella grimontii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 60 | 15°C: about 55% of maximal activity, 60°C: about 70% of maximal activity | Leminorella grimontii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 3.7 | alpha-D-galactose | pH 9.0, 50°C | Leminorella grimontii | |
| 21.3 | - |
ATP | pH 9.0, 50°C | Leminorella grimontii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Leminorella grimontii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 65% of maximal activity, pH 10.0: about 90% of maximal activity | Leminorella grimontii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.1 | - |
alpha-D-galactose | pH 9.0, 50°C | Leminorella grimontii | |
| 32.9 | - |
ATP | pH 9.0, 50°C | Leminorella grimontii | |
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Release 2023.1 (January 2023)
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