Literature summary for 2.7.1.6 extracted from

Lobo-Rojas, T.; Gonzalez-Marcano, E.; Valera-Vera, E.; Acosta, H.; Quinones, W.; Burchmore, R.; Concepcion, J.; Caceres, A.
Trypanosoma cruzi contains two galactokinases; molecular and biochemical characterization (2016), Parasitol. Int., 65, 472-482.

Search for more literature for 2.7.1.6

Activating Compound

Activating Compound	Comment	Organism	Structure
KCl	50-100 mM, about 80% increase in activity	Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	isoform GalK2; isoform GalK2	Trypanosoma cruzi
NaCl	50500 mM, the enzymatic activity decreases approximately 40%60%, respectively; 50500 mM, the enzymatic activity decreases approximately 40%60%, respectively	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.091	-	D-galactose	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi
0.1	-	ATP	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi
0.108	-	D-galactose	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi
0.36	-	ATP	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	both isoforms GalK1 and GalK2	Trypanosoma cruzi	20015	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	most effective divalent cation	Trypanosoma cruzi
Mn2+	62% of the activity with Mg2+	Trypanosoma cruzi
additional information	divalent cation required	Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	gel filtration, both isoforms GalK1 and GalK2	Trypanosoma cruzi
51300	-	-	Trypanosoma cruzi
51900	-	-	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q4DF18	isoform Galk1	-
Trypanosoma cruzi	Q4DFH6	isoform GALK2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
epimastigote	expression of both isoforms GalK1 and GalK2	Trypanosoma cruzi	-
trypomastigote	expression of both isoforms GalK1 and GalK2	Trypanosoma cruzi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-galactose	-	Trypanosoma cruzi	ADP + D-galactose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 51300, calculated, 1 * 50000, SDS-PAGE	Trypanosoma cruzi
monomer	1 * 51900, calculated, 1 * 50000, SDS-PAGE	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
CLB.507001.110	-	Trypanosoma cruzi
CLB.510667.120	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.38	-	D-galactose	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	9.5	-	Trypanosoma cruzi
7.5	9.5	isoform GalK1	Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.41	-	ATP	isoform GalK2, pH 8.6, temperature not specified in the publication	Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.5	-	D-galactose	pH 8.6, temperature not specified in the publication	Trypanosoma cruzi

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Release 2023.1 (January 2023)