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Literature summary for 2.7.1.6 extracted from
- A highly efficient galactokinase from Bifidobacterium infantis with broad substrate specificity (2012), Carbohydr. Res., 355, 35-39.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bifidobacterium longum subsp. infantis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.895 | - |
D-galactose | pH 8, 37°C | Bifidobacterium longum subsp. infantis |  |
| 2.46 | - |
ATP | pH 8, 37°C | Bifidobacterium longum subsp. infantis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bifidobacterium longum subsp. infantis | |
| Mn2+ | can partly substitute Mg2+, with the reduction of the activity by about 21% | Bifidobacterium longum subsp. infantis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Bifidobacterium longum subsp. infantis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum subsp. infantis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Bifidobacterium longum subsp. infantis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0781 | - |
substrate: ATP + 6-deoxy-D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 0.09 | - |
substrate: ATP + D-galacturonic acid, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 0.52 | - |
substrate: UTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 0.551 | - |
substrate: CTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 0.931 | - |
substrate: dCTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 1.5 | - |
substrate: dUTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 2.5 | - |
substrate: dTTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 12.5 | - |
substrate: ATP + D-galactosamine, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 15.7 | - |
substrate: ITP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 28.5 | - |
substrate: GTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 31.9 | - |
substrate: dGTP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 99.7 | - |
substrate: ATP + 2-deoxy-D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 137 | - |
substrate: ATP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 138 | - |
substrate: dATP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
| 158 | - |
substrate: ATP + D-galactose, pH 8, 37°C | Bifidobacterium longum subsp. infantis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-galactose | substrate: 2-deoxygalactose, specific activity: 99.7 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + 2-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
| ATP + 6-deoxy-D-galactose | substrate: 6-deoxygalactose, specific activity: 0.0781 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + 6-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | substrate: D-galactosamine, specific activity: 12.5 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactose | substrate: ATP, specific activity: 137 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactose | substrate: D-galactose, specific activity: 158 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galacturonic acid | substrate: galacturonic acid, specific activity: 0.09 micromol/min/mg | Bifidobacterium longum subsp. infantis | ADP + alpha-D-galacturonic acid 1-phosphate | - |
? | |
| CTP + D-galactose | substrate: CTP, specific activity: 0.551 micromol/min/mg | Bifidobacterium longum subsp. infantis | CDP + alpha-D-galactose 1-phosphate | - |
? | |
| dATP + D-galactose | substrate: dATP, specific activity: 138 micromol/min/mg | Bifidobacterium longum subsp. infantis | dADP + alpha-D-galactose 1-phosphate | - |
? | |
| dCTP + D-galactose | substrate: dCTP, specific activity: 0.931 micromol/min/mg | Bifidobacterium longum subsp. infantis | dCDP + alpha-D-galactose 1-phosphate | - |
? | |
| dGTP + D-galactose | substrate: dGTP, specific activity: 31.9 micromol/min/mg | Bifidobacterium longum subsp. infantis | dGDP + alpha-D-galactose 1-phosphate | - |
? | |
| dTTP + D-galactose | substrate: dTTP, specific activity: 2.5 micromol/min/mg | Bifidobacterium longum subsp. infantis | ? | - |
? | |
| dUTP + D-galactose | substrate: dUTP, specific activity: 1.5 micromol/min/mg | Bifidobacterium longum subsp. infantis | dUDP + alpha-D-galactose 1-phosphate | - |
? | |
| GTP + D-galactose | substrate: GTP, specific activity: 28.5 micromol/min/mg | Bifidobacterium longum subsp. infantis | GDP + alpha-D-galactose 1-phosphate | - |
? | |
| ITP + D-galactose | substrate: ITP, specific activity: 15.7 micromol/min/mg | Bifidobacterium longum subsp. infantis | IDP + alpha-D-galactose 1-phosphate | - |
? | |
| UTP + D-galactose | substrate: UTP, specific activity: 0.52 micromol/min/mg | Bifidobacterium longum subsp. infantis | UDP + alpha-D-galactose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BiGalK | - |
Bifidobacterium longum subsp. infantis |
| galactokinase | - |
Bifidobacterium longum subsp. infantis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bifidobacterium longum subsp. infantis |
| 55 | - |
highest activity | Bifidobacterium longum subsp. infantis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 30 | only 20% of maximal activity | Bifidobacterium longum subsp. infantis |
| 65 | - |
over 76% of maximal activity | Bifidobacterium longum subsp. infantis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 146 | - |
ATP | pH 8, 37°C | Bifidobacterium longum subsp. infantis | |
| 146 | - |
D-galactose | pH 8, 37°C | Bifidobacterium longum subsp. infantis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Bifidobacterium longum subsp. infantis |
| 8.5 | - |
optimal pH | Bifidobacterium longum subsp. infantis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | enzyme keeps over 60% of maximal activity | Bifidobacterium longum subsp. infantis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 59.5 | - |
ATP | pH 8, 37°C | Bifidobacterium longum subsp. infantis | |
| 164 | - |
D-galactose | pH 8, 37°C | Bifidobacterium longum subsp. infantis | |
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